Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis
Biol. Res
;
49: 1-14, 2016. ilus, graf
Article
in English
| LILACS
| ID: biblio-950868
ABSTRACT
BACKGROUND:
Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues.RESULTS:
We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 µM) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It also increased K+ efflux and altered the mRNA levels of Bax, Bcl-2, caspase-3, caspase-8, Fas, and p53.CONCLUSIONS:
Our results suggest that Cd2+ triggers the apoptosis of Siberian tiger fibroblasts by disturbing intracellular homeostasis. These results will aid in our understanding of the effects of Cd2+ on Siberian tigers and in developing interventions to treat and prevent cadmium poisoning.
Full text:
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Index:
LILACS (Americas)
Main subject:
Cadmium
/
Apoptosis
/
Intracellular Space
/
Tigers
/
Fibroblasts
/
Homeostasis
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2016
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Chinese Academy of Agricultural Sciences/CN
/
Jinzhou Medical University/CN
/
Mudanjiang Normal University/CN
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