The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway
Biol. Res
;
51: 14, 2018. graf
Article
in English
| LILACS
| ID: biblio-950900
ABSTRACT
BACKGROUND:
Neurokinin1 (NK1) receptor has played a vital role in the development of tumor. However, NKP608 as a NK1 receptor antagonist whether has the effect of the resistance of colorectal cancer is still unclear. Thereby, in this study, we investigated the role of NKP608 on human colorectal cancer and explored the underlying mechanism.METHODS:
The cell proliferation of colorectal cancer cells was detected by cell counting kit-8 (CCK8) assay, cell migration and invasion were assessed by transwell assay, the apoptotic ratio of cells was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide stained and flow cytometry. The involvement of molecular mechanisms was examined by western blot.RESULTS:
In this study, we found that NKP608 inhibited the proliferation, migration/invasion of HCT116 cells. In addition, NKP608 reduced expressions of Wnt-3a, ß-catenin, Cyclin D1, and (vascular endothelial growth factor) VEGF while induced expression of E-Cadherin. Furthermore, flow cytometry analyzed that NKP608 induced apoptosis of HCT116 cells, consistently, western blotting detecting of apoptosis-related proteins revealed that NKP608 downregulated Bcl-2 while upregulated Bax and Active-Caspase-3.CONCLUSIONS:
Taken together, our results demonstrated that NKP608 inhibited colorectal cancer cell proliferation, migration and invasion via suppressing the Wnt/ß-catenin signaling pathway. Therefore, NKP608 might represent a promising therapeutic agent in the treatment of colorectal cancer.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Piperidines
/
Quinolines
/
Colorectal Neoplasms
/
Cell Movement
/
Cell Proliferation
/
Wnt Signaling Pathway
/
Neurokinin-1 Receptor Antagonists
Limits:
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2018
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Beijing University of Chinese Medicine/CN
/
Shanghai Pudong New Area Zhoupu Hospital/CN
/
The First Hospital of Yulin City/CN
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