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Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation
Mustafa, Siti Ratina; Husaini, Ahmad; Hipolito, Cirilo Nolasco; Hussain, Hasnain; Suhaili, Nurshikin; Roslan, Hairul Azman.
  • Mustafa, Siti Ratina; Universiti Malaysia Sarawak. Sarawak. MY
  • Husaini, Ahmad; Universiti Malaysia Sarawak. Sarawak. MY
  • Hipolito, Cirilo Nolasco; Universiti Malaysia Sarawak. Sarawak. MY
  • Hussain, Hasnain; Universiti Malaysia Sarawak. Faculty of Resource Science and Technology. MY
  • Suhaili, Nurshikin; Universiti Malaysia Sarawak. Sarawak. MY
  • Roslan, Hairul Azman; Universiti Malaysia Sarawak. Sarawak. MY
Braz. arch. biol. technol ; 59: e16150632, 2016. tab, graf
Article in English | LILACS | ID: biblio-951403
ABSTRACT
Amylase is recognized as one of the important commercial enzymes. This group of enzymes has the ability in hydrolyzing starch into smaller oligosacharides. The present work aimed to determine the optimum fermentation conditions for maximum production of crude amylase enzyme by Aspergillus flavus NSH9 employing response surface methodology (RSM).Central composite design (CCD) was applied to determine the optimal fermentation condition with respect to the four main process parameters such as temperature, initial moisture content, pH and the incubation period. Solid state fermentation (SSF) was performed using 5.0 g of sago hampas inoculated with 1x107sporesmL-1following the experimental design obtained using CCD and further optimized by RSM. The initial moisture, pH and temperature showed significant effect on the amylase production (p<0.05). The maximum amylase activity produced was achieved and recorded as 1.055 ± 0.03U mL-1after four days of fermentation period with 100% (v/v) moisture holding capacity, pH 6.5 and temperature at 28°C. The optimum fermentation conditions for amylase production was determined with A. flavusNSH9 on sago hampas.


Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2016 Type: Article / Project document Affiliation country: Malaysia Institution/Affiliation country: Universiti Malaysia Sarawak/MY

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Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2016 Type: Article / Project document Affiliation country: Malaysia Institution/Affiliation country: Universiti Malaysia Sarawak/MY