Your browser doesn't support javascript.
loading
Effects of diabetes and hypertension on oral mucosa and tgfß1 salivary levels
Bernardi, Lisiane; Souza, Bárbara Capitanio de; Sonda, Nicole Canalli; Visioli, Fernanda; Rados, Pantelis Varvaki; Lamers, Marcelo Lazzaron.
  • Bernardi, Lisiane; Universidade Federal do Rio Grande do Sul. Basic Research Center, Dentistry School. Porto Alegre. BR
  • Souza, Bárbara Capitanio de; Universidade Federal do Rio Grande do Sul. Basic Research Center, Dentistry School. Porto Alegre. BR
  • Sonda, Nicole Canalli; Universidade Federal do Rio Grande do Sul. Basic Research Center, Dentistry School. Porto Alegre. BR
  • Visioli, Fernanda; Universidade Federal do Rio Grande do Sul. Department of Oral Pathology, Dentistry School. Porto Alegre. BR
  • Rados, Pantelis Varvaki; Universidade Federal do Rio Grande do Sul. Department of Oral Pathology, Dentistry School. Porto Alegre. BR
  • Lamers, Marcelo Lazzaron; Universidade Federal do Rio Grande do Sul. Department of Morphological Sciences. Porto Alegre. BR
Braz. dent. j ; 29(3): 309-315, May-June 2018. tab, graf
Article in English | LILACS | ID: biblio-951550
Responsible library: BR1.1
ABSTRACT
Abstract The aim of this study was to investigate salivary levels of TGFβ1 and proliferation/ maturation of epithelial mucosa cells in diabetic and hypertensive patients.

Design:

in this cross-sectional study, whole stimulated saliva and oral mucosa exfoliative cytology specimens were collected from 39 patients that were healthy (control, n=10) or presented history of arterial hypertension (HAS, n=9), diabetes mellitus (DM, n=10) or both (DM+HAS, n=10). Salivary flow rate (SFR), TGFβ1 level in saliva, AgNORs and the epithelial maturation were evaluated. Non-parametric Kruskal-Wallis test, followed by Dunn's multiple comparison post-test and the Spearman test correlation analysis were used. SFR showed a significant decreased in DM and DM+HAS (0.47±0.11 and 0.64±0.43 mL/min) when compared to control (1.4±0.38 mL/min). DM+HAS presented the highest value of TGFβ1 concentration (24.72±5.89 pg/mL). It was observed a positive correlation between TGFβ1 and glycaemia (R=0.6371; p<0.001) and a negative correlation between TGFβ1 and saliva (R=-0.6162; p<0.001) and glycaemia and SFR (R=-0.5654; P=0.001). AgNORs number and status of maturation of mucosa cells were similar for all conditions. DM and DM+HAS presented the lowest SFR, which correlated with increased TGFβ1 levels. Despite the higher TGFβ1 secretion it was not observed changes in the morphology or proliferation of epithelial cells when diabetes or hypertension was present.
RESUMO
Resumo O objetivo deste estudo foi investigar os níveis de TGFβ1 na saliva e a proliferação/maturação das células epiteliais da mucosa em paciente diabéticos e hipertensos. Neste estudo transversal, saliva estimulada e amostras de citologia exfoliativa de mucosa oral foram coletadas de um total de 39 pacientes que se apresentavam saudáveis (controle, n=10) ou com história de hipertensão arterial (HAS, n=9), diabetes mellitus (DM, n=10) ou ambos (DM+HAS, n=10). Taxa de fluxo salivar (SFR), níveis de TGFβ1 na saliva, AgNORs e maturação epitelial foram avaliados. Teste não-paramétrico de Kruskal-Wallis, seguido de comparação múltipla de Dunn e correlação de Spearman foram utilizados para as análises. SFR diminuiu significantemente em DM e DM+HAS (0,47±0,11 e 0,64±0,43 mL/min) quando comparado ao controle (1,4±0,38 mL/min). DM+HAS apresentou os maiores valores de concentração de TGFβ1 (24,72±5,89 pg/mL). Foi observada uma correlação positiva entre TGFβ1 e glicemia (R=0,6371; p<0,001) e uma correlação negativa entre TGFβ1 e saliva (R=-0,6162; p<0,001) e glicemia e SFR (R=-0,5654; p=0,001). Número de AgNORs e o padrão da maturação das células epiteliais foram similares entre os todos grupos. DM e DM+HAS apresentaram os menores valores de SFR, os quais foram correlacionados com o aumento nos níveis de TGFβ1. Apesar da maior secreção de TGFβ1, não foram observadas mudanças na morfologia ou proliferação das células epiteliais quando o paciente apresentava diabetes ou hipertensão.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Saliva / Diabetes Mellitus / Transforming Growth Factor beta1 / Hypertension / Mouth Mucosa Type of study: Prevalence study Limits: Adult / Female / Humans / Male Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR

Full text: Available Index: LILACS (Americas) Main subject: Saliva / Diabetes Mellitus / Transforming Growth Factor beta1 / Hypertension / Mouth Mucosa Type of study: Prevalence study Limits: Adult / Female / Humans / Male Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR