Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts
Braz. j. med. biol. res
;
49(4): e4324, 2016. tab, graf
Article
in English
| LILACS
| ID: biblio-951663
ABSTRACT
The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Periodontal Ligament
/
Interleukin-10
/
RANK Ligand
/
Osteoprotegerin
/
Fibroblasts
/
Glucose
Limits:
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2016
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Nanjing Medical University/CN
/
Xi'an Jiaotong University College of Medicine/CN
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