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Transplantation and survival of mouse inner ear progenitor/stem cells in the organ of Corti after cochleostomy of hearing-impaired guinea pigs: preliminary results
Barboza Jr, L C M; Lezirovitz, K; Zanatta, D B; Strauss, B E; Mingroni-Netto, R C; Oiticica, J; Haddad, L A; Bento, R F.
  • Barboza Jr, L C M; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Departamento de Otorrinolaringologia (LIM32). São Paulo. BR
  • Lezirovitz, K; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Departamento de Otorrinolaringologia (LIM32). São Paulo. BR
  • Zanatta, D B; Universidade de São Paulo. Instituto do Coração, Faculdade de Medicina. Laboratório de Genética e Cardiologia Molecular. Setor de Vetores Virais. São Paulo. BR
  • Strauss, B E; Universidade de São Paulo. Instituto do Coração. Laboratório de Genética e Cardiologia Molecular. São Paulo. BR
  • Mingroni-Netto, R C; Universidade de São Paulo. Instituto de Biociências. Departamento de Genética e Biologia Evolutiva. São Paulo. BR
  • Oiticica, J; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Haddad, L A; Universidade de São Paulo. Instituto de Biociências. Departamento de Genética e Biologia Evolutiva. São Paulo. BR
  • Bento, R F; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Departamento de Otorrinolaringologia (LIM32). São Paulo. BR
Braz. j. med. biol. res ; 49(4): e5064, 2016. tab, graf
Article in English | LILACS | ID: biblio-951670
Responsible library: BR1.1
ABSTRACT
In mammals, damage to sensory receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing loss. Here, we investigated whether postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after transplantation into the basal turns of neomycin-injured guinea pig cochleas. We also examined the effects of mIESC transplantation on auditory functions. Eight adult female Cavia porcellus guinea pigs (250-350g) were deafened by intratympanic neomycin delivery. After 7 days, the animals were randomly divided in two groups. The study group (n=4) received transplantation of LacZ-positive mIESCs in culture medium into the scala tympani. The control group (n=4) received culture medium only. At 2 weeks after transplantation, functional analyses were performed by auditory brainstem response measurement, and the animals were sacrificed. The presence of mIESCs was evaluated by immunohistochemistry of sections of the cochlea from the study group. Non-parametric tests were used for statistical analysis of the data. Intratympanic neomycin delivery damaged hair cells and increased auditory thresholds prior to cell transplantation. There were no significant differences between auditory brainstem thresholds before and after transplantation in individual guinea pigs. Some mIESCs were observed in all scalae of the basal turns of the injured cochleas, and a proportion of these cells expressed the hair cell marker myosin VIIa. Some transplanted mIESCs engrafted in the cochlear basilar membrane. Our study demonstrates that transplanted cells survived and engrafted in the organ of Corti after cochleostomy.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Organ of Corti / Stem Cells / Stem Cell Transplantation / Hair Cells, Auditory, Inner / Hearing Loss, Sensorineural Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

Full text: Available Index: LILACS (Americas) Main subject: Organ of Corti / Stem Cells / Stem Cell Transplantation / Hair Cells, Auditory, Inner / Hearing Loss, Sensorineural Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR