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Macelignan inhibits bee pathogenic fungi Ascophaera apis growth through HOG1 pathway
Shin, Y K; Kim, K Y.
  • Shin, Y K; Kyung Hee University. College of Life Science. Yongin-si. KR
  • Kim, K Y; Kyung Hee University. Department of Genetic Engineering. Life Science and Graduate School of Biotechnology. Yongin-si. KR
Braz. j. med. biol. res ; 49(7): e5313, 2016. tab, graf
Article in English | LILACS | ID: biblio-951690
Responsible library: BR1.1
ABSTRACT
Ascosphaera apis is a bee pathogen that causes bee larvae infection disease, to which treatment is not yet well investigated. The aim of this study was to investigate antifungal susceptibility in vitro against A. apis and to identify a new antifungal agent for this pathogen through minimal inhibitory concentration (MIC) assay and western blot analysis. Macelignan had 1.56 and 3.125 μg/mL MIC against A. apis after 24 and 48 h, respectively, exhibiting the strongest growth inhibition against A. apis among the tested compounds (corosolic acid, dehydrocostus lactone, loganic acid, tracheloside, fangchinoline and emodin-8-O-β-D-glucopyranoside). Furthermore, macelignan showed a narrow-ranged spectrum against various fungal strains without any mammalian cell cytotoxicity. In spite of miconazole having powerful broad-ranged anti-fungal activity including A. apis, it demonstrated strong cytotoxicity. Therefore, even if macelignan alone was effective as an antifungal agent to treat A. apis, combined treatment with miconazole was more useful to overcome toxicity, drug resistance occurrence and cost effectiveness. Finally, HOG1 was revealed as a target molecule of macelignan in the anti-A. apis activity by inhibiting phosphorylation using S. cerevisiae as a model system. Based on our results, macelignan, a food-grade antimicrobial compound, would be an effective antifungal agent against A. apis infection in bees.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Ascomycota / Bees / Lignans / Mitogen-Activated Protein Kinases / Saccharomyces cerevisiae Proteins / Antifungal Agents Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article Affiliation country: South Korea Institution/Affiliation country: Kyung Hee University/KR

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Full text: Available Index: LILACS (Americas) Main subject: Ascomycota / Bees / Lignans / Mitogen-Activated Protein Kinases / Saccharomyces cerevisiae Proteins / Antifungal Agents Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article Affiliation country: South Korea Institution/Affiliation country: Kyung Hee University/KR