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Quercetin alleviates pulmonary angiogenesis in a rat model of hepatopulmonary syndrome
Li, X; Chen, Y; Wang, L; Shang, G; Zhang, C; Zhao, Z; Zhang, H; Liu, A.
  • Li, X; Changzhi Medical College. Department of Physiology. Changzhi. CN
  • Chen, Y; Changzhi Medical College. Department of Microbiology. Changzhi. CN
  • Wang, L; Functional Laboratory of Changzhi Medical College. Changzhi. CN
  • Shang, G; Changzhi Medical College. Department of Physiology. Changzhi. CN
  • Zhang, C; Changzhi Medical College. Department of Physiology. Changzhi. CN
  • Zhao, Z; Liver Disease Institute of Changzhi Medical College. Changzhi. CN
  • Zhang, H; Changzhi Medical College. Department of Physiology. Changzhi. CN
  • Liu, A; China Academy of Chinese Medical Sciences. Institute of Chinese Materia Medica. Beijing. CN
Braz. j. med. biol. res ; 49(7): e5326, 2016. graf
Article in English | LILACS | ID: biblio-951692
ABSTRACT
Quercetin shows protective effects against hepatopulmonary syndrome (HPS), as demonstrated in a rat model. However, whether these effects involve pulmonary vascular angiogenesis in HPS remains unclear. Therefore, this study aimed to assess the effect of quercetin on pulmonary vascular angiogenesis and explore the underlying mechanisms. Male Sprague-Dawley rats weighing 200-250 g underwent sham operation or common bile duct ligation (CBDL). Two weeks after surgery, HIF-1α and NFκB levels were assessed in rat lung tissue by immunohistochemistry and western blot. Then, CBDL and sham-operated rats were further divided into 2 subgroups each to receive intraperitoneal administration of quercetin (50 mg/kg daily) or 0.2% Tween for two weeks Sham (Sham+Tween; n=8), CBDL (CBDL+Tween; n=8), Q (Sham+quercetin; n=8), and CBDL+Q (CBDL+quercetin; n=8). After treatment, lung tissue specimens were assessed for protein (immunohistochemistry and western blot) and/or gene expression (quantitative real-time PCR) levels of relevant disease markers, including VEGFA, VEGFR2, Akt/p-Akt, HIF-1α, vWf, and IκB/p-IκB. Finally, arterial blood was analyzed for alveolar arterial oxygen pressure gradient (AaPO2). Two weeks after CBDL, HIF-1α expression in the lung decreased, but was gradually restored at four weeks. Treatment with quercetin did not significantly alter HIF-1α levels, but did reduce AaPO2 as well as lung tissue NF-κB activity, VEGFA gene and protein levels, Akt activity, and angiogenesis. Although hypoxia is an important feature in HPS, our findings suggest that HIF-1α was not the main cause for the VEGFA increase. Interestingly, quercetin inhibited pulmonary vascular angiogenesis in rats with HPS, with involvement of Akt/NF-κB and VEGFA/VEGFR-2 pathways.
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Full text: Available Index: LILACS (Americas) Main subject: Hepatopulmonary Syndrome / Lung / Neovascularization, Pathologic / Antioxidants Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article Affiliation country: China Institution/Affiliation country: Changzhi Medical College/CN / China Academy of Chinese Medical Sciences/CN / Functional Laboratory of Changzhi Medical College/CN / Liver Disease Institute of Changzhi Medical College/CN

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Full text: Available Index: LILACS (Americas) Main subject: Hepatopulmonary Syndrome / Lung / Neovascularization, Pathologic / Antioxidants Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article Affiliation country: China Institution/Affiliation country: Changzhi Medical College/CN / China Academy of Chinese Medical Sciences/CN / Functional Laboratory of Changzhi Medical College/CN / Liver Disease Institute of Changzhi Medical College/CN