Your browser doesn't support javascript.
loading
Evaluation of dot-blot test for serological diagnosis of bovine brucellosis
Bastos, Carla Resende; Mathias, Luis Antonio; Jusi, Márcia Mariza Gomes; Santos, Renata Ferreira dos; Silva, Glaucenyra Cecília Pinheiro da; André, Marcos Rogério; Machado, Rosangela Zacarias; Bürger, Karina Paes.
  • Bastos, Carla Resende; São Paulo State University. School of Agricultural and Veterinarian Sciences. Department of Preventive Veterinary Medicine and Animal Reproduction. Jaboticabal. BR
  • Mathias, Luis Antonio; São Paulo State University. School of Agricultural and Veterinarian Sciences. Department of Preventive Veterinary Medicine and Animal Reproduction. Jaboticabal. BR
  • Jusi, Márcia Mariza Gomes; São Paulo State University. School of Agricultural and Veterinarian Sciences. Department of Veterinary Pathology. Jaboticabal. BR
  • Santos, Renata Ferreira dos; São Paulo State University. School of Agricultural and Veterinarian Sciences. Department of Preventive Veterinary Medicine and Animal Reproduction. Jaboticabal. BR
  • Silva, Glaucenyra Cecília Pinheiro da; São Paulo State University. School of Agricultural and Veterinarian Sciences. Department of Preventive Veterinary Medicine and Animal Reproduction. Jaboticabal. BR
  • André, Marcos Rogério; São Paulo State University. School of Agricultural and Veterinarian Sciences. Department of Veterinary Pathology. Jaboticabal. BR
  • Machado, Rosangela Zacarias; São Paulo State University. School of Agricultural and Veterinarian Sciences. Department of Veterinary Pathology. Jaboticabal. BR
  • Bürger, Karina Paes; São Paulo State University. School of Agricultural and Veterinarian Sciences. Department of Preventive Veterinary Medicine and Animal Reproduction. Jaboticabal. BR
Braz. j. microbiol ; 49(3): 564-568, July-Sept. 2018. tab, graf
Article in English | LILACS | ID: biblio-951797
ABSTRACT
Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1100, and the conjugate at 130,000. The comparison of the dot-blot results with 2-ME showed Kappa index of 0.9939, sensitivity of 99.48%, and specificity 99.91%, with CF, Kappa index of 0.8226, sensitivity 100% and specificity 95.32%. Using the combination of the test results 2-ME and CF to establish the true condition of the animal, the dot-blot showed relative sensitivity of 100%, and relative specificity of 99.91%. The evaluated test proved to be effective and reliable, besides being easy to handle and interpret the results.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Brucella abortus / Brucellosis / Serologic Tests / Cattle Diseases / Antibodies, Bacterial Type of study: Diagnostic study / Evaluation studies Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: São Paulo State University/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Brucella abortus / Brucellosis / Serologic Tests / Cattle Diseases / Antibodies, Bacterial Type of study: Diagnostic study / Evaluation studies Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: São Paulo State University/BR