Evaluation of dot-blot test for serological diagnosis of bovine brucellosis
Braz. j. microbiol
;
49(3): 564-568, July-Sept. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-951797
ABSTRACT
Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1100, and the conjugate at 130,000. The comparison of the dot-blot results with 2-ME showed Kappa index of 0.9939, sensitivity of 99.48%, and specificity 99.91%, with CF, Kappa index of 0.8226, sensitivity 100% and specificity 95.32%. Using the combination of the test results 2-ME and CF to establish the true condition of the animal, the dot-blot showed relative sensitivity of 100%, and relative specificity of 99.91%. The evaluated test proved to be effective and reliable, besides being easy to handle and interpret the results.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Brucella abortus
/
Brucellosis
/
Serologic Tests
/
Cattle Diseases
/
Antibodies, Bacterial
Type of study:
Diagnostic study
/
Evaluation studies
Limits:
Animals
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
São Paulo State University/BR
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