Application of n-dodecane as an oxygen vector to enhance the activity of fumarase in recombinant Escherichia coli: role of intracellular microenvironment
Braz. j. microbiol
;
49(3): 662-667, July-Sept. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-951808
ABSTRACT
Abstract The effect of the intracellular microenvironment in the presence of an oxygen vector during expression of a fusion protein in Escherichia coli was studied. Three organic solutions at different concentration were chosen as oxygen vectors for fumarase expression. The addition of n-dodecane did not induce a significant change in the expression of fumarase, while the activity of fumarase increased significantly to 124% at 2.5% n-dodecane added after 9 h induction. The concentration of ATP increased sharply during the first 6 h of induction, to a value 7600% higher than that in the absence of an oxygen-vector. NAD/NADH and NADP/NADPH ratios were positively correlated with fumarase activity. n-Dodecane can be used to increase the concentration of ATP and change the energy metabolic pathway, providing sufficient energy for fumarase folding.
Full text:
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Index:
LILACS (Americas)
Main subject:
Oxygen
/
Gene Expression
/
Alkanes
/
Escherichia coli
/
Fumarate Hydratase
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2018
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Nanjing University of Chinese Medicine/CN
/
Nanjing University of Technology/CN
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