Phenolic content and antibacterial activity of extracts of Hamelia patens obtained by different extraction methods
Braz. j. microbiol
;
49(3): 656-661, July-Sept. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-951809
ABSTRACT
Abstract Hamelia patens, is a plant traditionally used to treat a variety of conditions among the Huastec people of Mexico. The objective of this study is to characterize the phenolic content and critically examine the antimicrobial activity of leaf extracts H. patens, obtained by maceration, Soxhlet and percolation, using ethanol as 70% solvent. Phenolic compounds are characterized by liquid chromatography, coupled to a High Resolution Mass Spectrometry, and the antimicrobial activity was studied from the inhibitory effect of each extract for Escherichia coli, Staphylococcus aureus, Salmonella typhi and S. paratyphi, and by the Minimum Bactericidal Concentration, the percentage of activity and the Index of Bacterial Susceptibility of each extract. The phenolic compound identified in different concentrations in the three extracts was epicatechin. The extracts obtained by the three methods had antimicrobial activity, however, there was no significant difference (p < 0.05) between the Minimum Bactericidal Concentration of the extracts obtained by maceration, percolation and Soxhlet. The results of this study contribute to the body of knowledge on the use of extracts in controlling microorganisms with natural antimicrobials.
Full text:
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Index:
LILACS (Americas)
Main subject:
Phenols
/
Plant Extracts
/
Hamelia
/
Chemical Fractionation
/
Anti-Bacterial Agents
Type of study:
Evaluation studies
Country/Region as subject:
Mexico
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2018
Type:
Article
Affiliation country:
Mexico
Institution/Affiliation country:
Tecnológico Nacional de México/MX
/
Universidad Autónoma de Coahuila/MX
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