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Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis
Rungrattanakasin, Budsayachat; Premjet, Siripong; Thanonkeo, Sudarat; Klanrit, Preekamol; Thanonkeo, Pornthap.
  • Rungrattanakasin, Budsayachat; Khon Kaen University. Faculty of Technology. Department of Biotechnology. Khon Kaen. TH
  • Premjet, Siripong; Naresuan University. Faculty of Science. Department of Biology. Phitsanulok. TH
  • Thanonkeo, Sudarat; Mahasarakham University. Walai Rukhavej Botanical Research Institute. Maha Sarakham. TH
  • Klanrit, Preekamol; Khon Kaen University. Faculty of Technology. Department of Biotechnology. Khon Kaen. TH
  • Thanonkeo, Pornthap; Khon Kaen University. Faculty of Technology. Department of Biotechnology. Khon Kaen. TH
Braz. j. microbiol ; 49(3): 647-655, July-Sept. 2018. graf
Article in English | LILACS | ID: biblio-951810
ABSTRACT
Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and encoded a protein of 460 amino acid residues. The predicted molecular weight and the isoelectric point of the A. fumigatus Cel7 gene product were 48.19 kDa and 5.03, respectively. A catalytic domain in the N-terminal region and a fungal type cellulose-binding domain/module in the C-terminal region were detected in the predicted polypeptide sequences. Furthermore, a signal peptide with 20 amino acid residues at the N-terminus was also detected in the deduced amino acid sequences of the endoglucanase from A. fumigatus DBiNU-1. The endoglucanase from A. fumigatus DBiNU-1 was successfully expressed in K. lactis, and the purified recombinant enzyme exhibited its maximum activity at pH 5.0 and 60 °C. The enzyme was very stable in a pH range from 4.0 to 8.0 and a temperature range from 30 to 60 °C. These features make it suitable for application in the paper, biofuel, and other chemical production industries that use cellulosic materials.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Aspergillus fumigatus / Fungal Proteins / Gene Expression / Cellulase / Cloning, Molecular Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Thailand Institution/Affiliation country: Khon Kaen University/TH / Mahasarakham University/TH / Naresuan University/TH

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Full text: Available Index: LILACS (Americas) Main subject: Aspergillus fumigatus / Fungal Proteins / Gene Expression / Cellulase / Cloning, Molecular Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Thailand Institution/Affiliation country: Khon Kaen University/TH / Mahasarakham University/TH / Naresuan University/TH