Astrocytic expression of GFAP and serum levels of IL-1ß and TNF-α in rats treated with different pain relievers
Braz. j. pharm. sci
;
52(4): 623-633, Oct.-Dec. 2016. graf
Article
in English
| LILACS
| ID: biblio-951871
ABSTRACT
ABSTRACT Pro-inflammatory cytokines and glial cells, especially microglial cells, have been implicated in persistent pain sensitization. Less is known about the role of astrocytes in pain regulation. This study aimed to observe the expression of the astrocytic biomarker glial fibrillary acidic protein (GFAP) and the serum levels of interleukin 1 beta (IL-1ß) and tumor necrosis factor alpha (TNF-α) after short-term administration of central pain relievers in rats not submitted to noxious stimuli. Male Wistar rats were divided into five groups, receiving for nine days- (1) amitriptyline (Amt-10 mg/kg/day, by gavage); (2) gabapentin (Gb-60 mg/kg/day, by gavage; (3) methadone (Me-4.5 mg/kg/day, intraperitoneal route [IP]); (4) morphine (Mo-10 mg/kg/day, IP); or (5) 0.9% saline solution, IP. Brain samples were collected for immunohistochemical study of GFAP expression in the mesencephalon and nucleus accumbens (NAc). The area of GFAP-positive cells was calculated using MetaMorphï software and serum levels of IL-1ß and TNF-α were measured by enzyme-linked immunosorbent assay. Serum TNF-α levels were decreased in the groups treated with Mo, Me and Gb, but not in the Amt-treated group. IL-1ß decreased only in rats treated with Me. The astrocytic expression of GFAP was decreased in the brainstem with all drugs, while it was increased in the NAc with Amt, Me and Mo
Full text:
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Index:
LILACS (Americas)
Main subject:
Glial Fibrillary Acidic Protein
/
Analgesics
Limits:
Animals
Language:
English
Journal:
Braz. j. pharm. sci
Year:
2016
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of São Paulo/BR
/
University Cruzeiro do Sul/BR
/
University Paulista/BR
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