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Augmented cytotoxic, mutagenic and genotoxic response triggered by carvedilol and celecoxib combinations
Attiq, Ali; Ashraf, Muhammad; Jalil, Juriyati; Javeed, Aqeel; Anjum, Aftab Ahmad; Ullah, Asad; Umair, Muhammad; Ali, Sarwat.
  • Attiq, Ali; Universiti Kebangsaan Malaysia. Faculty of Pharmacy. Drug and Herbal Research Center. Jalan Raja Muda Abdul Aziz. MY
  • Ashraf, Muhammad; University of Veterinary and Animal Sciences. Department Of Pharmacology and Toxicology. Lahore. PK
  • Jalil, Juriyati; Universiti Kebangsaan Malaysia. Faculty of Pharmacy. Drug and Herbal Research Center. Jalan Raja Muda Abdul Aziz. MY
  • Javeed, Aqeel; University of Veterinary and Animal Sciences. Department Of Pharmacology and Toxicology. Lahore. PK
  • Anjum, Aftab Ahmad; University of Veterinary and Animal Science. Department of Microbiology. Lahore. PK
  • Ullah, Asad; University of Veterinary and Animal Sciences. Department Of Pharmacology and Toxicology. Lahore. PK
  • Umair, Muhammad; University of Veterinary and Animal Sciences. Department Of Pharmacology and Toxicology. Lahore. PK
  • Ali, Sarwat; University of Veterinary and Animal Sciences. Department Of Pharmacology and Toxicology. Lahore. PK
Braz. J. Pharm. Sci. (Online) ; 54(1): e17292, 2018. tab, graf
Article in English | LILACS | ID: biblio-951918
ABSTRACT
It is understood that drugs regardless of their order of administration can exhibit drug interactions. Established on the fact that treatment of hypertension may last for decades and prolong usage of multiple drug regimen may induce substantial pathophysiological changes. Hence, This study was designed to evaluate the possible synergistic toxic effects of anti-hypertensive (carvedilol), and anti-inflammatory drug (celecoxib) alone and in combinations. Well-established MTT assay, Single Cell Gel Electrophoresis (SCGE) and Ames assay were employed to evaluate the toxicity at cellular level. Results from MTT assay on Vero cell line revealed that drug combinations have more pronounced anti-proliferative activity with combine IC50 value of 13.747.8 µg/mL. Likewise, exposure of peripheral blood mononuclear cells with drug combinations revealed significant (P<0.05) DNA damage (Class 3) in a dose dependent manner at concentrations ≥ 0.78 2.34 µg/mL. However, carvedilol and celecoxib were non mutagenic against either mutant strain (TA 100 and TA 98) and combinations have also shown mild to moderate mutagenic potential. Nevertheless, upon addition of metabolic activation enzyme, concentration <12.537.5 µg/plate exhibited significant (P<0.05) mutagenicity against both tester strains. In conclusion, this study provides additional genotoxicity and mutagenicity data that could be used in considering options for formulating regimens with reduced mutagenic potential
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Full text: Available Index: LILACS (Americas) Main subject: Celecoxib / Anti-Inflammatory Agents / Mutagenicity Tests / Antihypertensive Agents Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2018 Type: Article Affiliation country: Malaysia / Pakistan Institution/Affiliation country: Universiti Kebangsaan Malaysia/MY / University of Veterinary and Animal Science/PK / University of Veterinary and Animal Sciences/PK

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Full text: Available Index: LILACS (Americas) Main subject: Celecoxib / Anti-Inflammatory Agents / Mutagenicity Tests / Antihypertensive Agents Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2018 Type: Article Affiliation country: Malaysia / Pakistan Institution/Affiliation country: Universiti Kebangsaan Malaysia/MY / University of Veterinary and Animal Science/PK / University of Veterinary and Animal Sciences/PK