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Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
Utescher, Carla Lilian de Agostini; Buosi, Klariane Laís; Botosso, Viviane Fungaro; Quintilio, Wagner.
  • Utescher, Carla Lilian de Agostini; Instituto Butantan. Virology Laboratory. São Paulo. BR
  • Buosi, Klariane Laís; Merck SA. São Paulo. BR
  • Botosso, Viviane Fungaro; Instituto Butantan. Virology Laboratory. São Paulo. BR
  • Quintilio, Wagner; Instituto Butantan. Laboratory of Biopharmaceuticals in animal cells. São Paulo. BR
Braz. J. Pharm. Sci. (Online) ; 54(2): e17530, 2018. tab, graf
Article in English | LILACS | ID: biblio-951929
ABSTRACT
ABSTRACT The use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are suitable. Kit transport and storage conditions were also evaluated, proving that an adequate cold chain must be assured to achieve good results. Furthermore, the commercial MAT kit seemed suitable to replace the rabbit pyrogen test (RPT) for pyrogen testing of hyperimmune sera, although further tests are needed to a full validation.
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Full text: Available Index: LILACS (Americas) Main subject: Pyrogens / Serum Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2018 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Instituto Butantan/BR / Merck SA/BR

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Full text: Available Index: LILACS (Americas) Main subject: Pyrogens / Serum Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2018 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Instituto Butantan/BR / Merck SA/BR