Your browser doesn't support javascript.
loading
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses
Melo, Tiago André Fontoura de; Gründling, Grasiela Sabrina Longhi; Montagner, Francisco; Scur, Alcione Luiz; Steier, Liviu; Scarparo, Roberta Kochenborger; Figueiredo, José Antônio Poli de; Vier-Pelisser, Fabiana Vieira.
  • Melo, Tiago André Fontoura de; Pontifícia Universidade Católica do Rio Grande do Sul. School of Dentistry. Clinical Department. Porto Alegre. BR
  • Gründling, Grasiela Sabrina Longhi; Pontifícia Universidade Católica do Rio Grande do Sul. School of Dentistry. Clinical Department. Porto Alegre. BR
  • Montagner, Francisco; Pontifícia Universidade Católica do Rio Grande do Sul. School of Dentistry. Porto Alegre. BR
  • Scur, Alcione Luiz; s.af
  • Steier, Liviu; University of Warwick. Warwick Medical School. Institute of Clinical Education. Coventry. GB
  • Scarparo, Roberta Kochenborger; Pontifícia Universidade Católica do Rio Grande do Sul. School of Dentistry. Clinical Department. Porto Alegre. BR
  • Figueiredo, José Antônio Poli de; Pontifícia Universidade Católica do Rio Grande do Sul. School of Dentistry. Clinical Department. Porto Alegre. BR
  • Vier-Pelisser, Fabiana Vieira; Pontifícia Universidade Católica do Rio Grande do Sul. School of Dentistry. Clinical Department. Porto Alegre. BR
Braz. oral res. (Online) ; 30(1): e19, 2016. tab, graf
Article in English | LILACS | ID: biblio-951989
ABSTRACT
Abstract The present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia colilipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used OZY® System, one 120-second-pulse (OZY 1p); OZY® System, four 24-second-pulses (OZY 4p); and Endox® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Oxidants, Photochemical / Ozone / Lipopolysaccharides / Dental Pulp Cavity / Electrocoagulation / Endotoxins / Escherichia coli Type of study: Controlled clinical trial / Evaluation studies Limits: Humans Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: Brazil / United kingdom Institution/Affiliation country: Pontifícia Universidade Católica do Rio Grande do Sul/BR / University of Warwick/GB

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Oxidants, Photochemical / Ozone / Lipopolysaccharides / Dental Pulp Cavity / Electrocoagulation / Endotoxins / Escherichia coli Type of study: Controlled clinical trial / Evaluation studies Limits: Humans Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: Brazil / United kingdom Institution/Affiliation country: Pontifícia Universidade Católica do Rio Grande do Sul/BR / University of Warwick/GB