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Suppressor of cytokine signaling 1 expression during LPS-induced inflammation and bone loss in rats
Souza, João Antonio Chaves de; Nogueira, Andressa Vilas Boas; Souza, Pedro Paulo Chaves de; Oliveira, Guilherme José Pimentel Lopes de; Medeiros, Marcell Costa de; Garlet, Gustavo Pompermaier; Cirelli, Joni Augusto; Rossa-Junior, Carlos.
  • Souza, João Antonio Chaves de; Universidade Federal de Goiás. Faculty of Dentistry. Department of Periodontology. Goiânia. BR
  • Nogueira, Andressa Vilas Boas; Universidade Estadual Paulista. School of Dentistry at Araraquara. Department of Diagnosis and Surgery. Araraquara. BR
  • Souza, Pedro Paulo Chaves de; Universidade Estadual Paulista. School of Dentistry at Araraquara. Department of Physiology and Pathology. Araraquara. BR
  • Oliveira, Guilherme José Pimentel Lopes de; Universidade Estadual Paulista. School of Dentistry at Araraquara. Department of Diagnosis and Surgery. Araraquara. BR
  • Medeiros, Marcell Costa de; Universidade Estadual Paulista. School of Dentistry at Araraquara. Department of Diagnosis and Surgery. Araraquara. BR
  • Garlet, Gustavo Pompermaier; Universidade de São Paulo. School of Dentistry at Bauru. Department of Biological Sciences. Bauru. BR
  • Cirelli, Joni Augusto; Universidade Estadual Paulista. School of Dentistry at Araraquara. Department of Diagnosis and Surgery. Araraquara. BR
  • Rossa-Junior, Carlos; Universidade Estadual Paulista. School of Dentistry at Araraquara. Department of Diagnosis and Surgery. Araraquara. BR
Braz. oral res. (Online) ; 31: e75, 2017. tab, graf
Article in English | LILACS | ID: biblio-952120
ABSTRACT
Abstract This study aimed to characterize the dynamics of suppressor of cytokine signaling (SOCS1) expression in a rat model of lipopolysaccharide-induced periodontitis. Wistar rats in the experimental groups were injected three times/week with LPS from Escherichia coli on the palatal aspect of the first molars, and control animals were injected with vehicle (phosphate-buffered saline). Animals were sacrificed 7, 15, and 30 days after the first injection to analyze inflammation (stereometric analysis), bone loss (macroscopic analysis), gene expression (qRT-PCR), and protein expression/activation (Western blotting). The severity of inflammation and bone loss associated with LPS-induced periodontitis increased from day 7 to day 15, and it was sustained through day 30. Significant (p < 0.05) increases in SOCS1, RANKL, OPG, and IFN-γ gene expression were observed in the experimental group versus the control group at day 15. SOCS1 protein expression and STAT1 and NF-κB activation were increased throughout the 30-day experimental period. Gingival tissues affected by experimental periodontitis express SOCS1, indicating that this protein may potentially downregulate signaling events involved in inflammatory reactions and bone loss and thus may play a relevant role in the development and progression of periodontal disease.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Alveolar Bone Loss / Suppressor of Cytokine Signaling 1 Protein Type of study: Etiology study / Prognostic study Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Federal de Goiás/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Alveolar Bone Loss / Suppressor of Cytokine Signaling 1 Protein Type of study: Etiology study / Prognostic study Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2017 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Federal de Goiás/BR / Universidade de São Paulo/BR