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Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
Lemos, Natália Emerim; Farias, Mariela Granero; Kubaski, Francyne; Scotti, Luciana; Onsten, Tor Gunnar Hugo; Brondani, Letícia de Almeida; Wagner, Sandrine Comparsi; Sekine, Leo.
  • Lemos, Natália Emerim; Feevale. Novo Hamburgo. BR
  • Farias, Mariela Granero; Hospital de Clínicas de Porto Alegre. Porto Alegre. BR
  • Kubaski, Francyne; Hospital de Clínicas de Porto Alegre. Porto Alegre. BR
  • Scotti, Luciana; Hospital de Clínicas de Porto Alegre. Porto Alegre. BR
  • Onsten, Tor Gunnar Hugo; Hospital de Clínicas de Porto Alegre. Porto Alegre. BR
  • Brondani, Letícia de Almeida; Hospital de Clínicas de Porto Alegre. Porto Alegre. BR
  • Wagner, Sandrine Comparsi; Feevale. Novo Hamburgo. BR
  • Sekine, Leo; Hospital de Clínicas de Porto Alegre. Porto Alegre. BR
Hematol., Transfus. Cell Ther. (Impr.) ; 40(3): 213-218, July-Sept. 2018. tab, graf, ilus
Article in English | LILACS | ID: biblio-953842
ABSTRACT
ABSTRACT

Background:

Due to laboratory logistic issues, our center has traditionally scheduled peripheral blood stem cell harvests based on timing from the start of mobilization. This has proved to be useful in some cases, but also resulted in many fruitless harvests due to poor mobilization. In order to improve the efficiency of collections and compare the effectiveness of peripheral blood CD34+ cells as a predictor with data from other reports, this study analyzed the implementation of this routine.

Methods:

Peripheral blood and leukapheresis samples were quantified by flow cytometry and the association between these parameters was assessed.

Results:

Sixty-six consecutive leukapheresis samples were collected from 34 patients after the collection of peripheral blood samples for CD34+ quantification. A moderate positive correlation was observed between peripheral blood CD34+ cell count and total CD34+ cell count/kg (r = 0.596; p-value < 0.001). A multivariable regression model also confirmed this association and allowed the estimation that for every increase in five CD34+ cells/µL in the peripheral blood, a mean increase of 0.38 × 106 CD34+ cells/kg could be predicted. Demographic characteristics, baseline comorbidities and mobilization regimen did not influence final CD34+ cell count in this sample.

Conclusions:

As observed in other centers, quantification of peripheral blood CD34+ progenitor cells is a strong predictor of effectiveness to guide stem cell harvesting. Due to the results of this study, a modification in the peripheral blood stem cell harvesting logistics was implemented at our center in order to incorporate this routine.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Stem Cells / Blood Component Removal / Antigens, CD34 / Flow Cytometry Type of study: Prognostic study Limits: Female / Humans / Male Language: English Journal: Hematol., Transfus. Cell Ther. (Impr.) Journal subject: Hematologia / TransfusÆo de Sangue Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Feevale/BR / Hospital de Clínicas de Porto Alegre/BR

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Full text: Available Index: LILACS (Americas) Main subject: Stem Cells / Blood Component Removal / Antigens, CD34 / Flow Cytometry Type of study: Prognostic study Limits: Female / Humans / Male Language: English Journal: Hematol., Transfus. Cell Ther. (Impr.) Journal subject: Hematologia / TransfusÆo de Sangue Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Feevale/BR / Hospital de Clínicas de Porto Alegre/BR