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Antioxidant capacity of Momordica charantia extract and its protective effect on testicular damage in valproic acid-induced rats / Capacidad antioxidante del extracto de Momordica charantia y su efecto protector sobre el daño testicular en ratas inducidas con ácido valproico
Maneenin, Chanwit; Burawat, Jaturon; Maneenin, Naowarat; Nualkaew, Somsak; Arun, Supatcharee; Sampannang, Apichakan; Iamsaard, Sitthichai.
  • Maneenin, Chanwit; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Burawat, Jaturon; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Maneenin, Naowarat; Khon Kaen University. Faculty of Public health. Department of Epidemiology and Biostatistics. Khon Kaen. TH
  • Nualkaew, Somsak; Mahasarakham University. Faculty of Pharmacy. Department of Pharmaceutical Sciences. Mahasarakham. TH
  • Arun, Supatcharee; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Sampannang, Apichakan; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Iamsaard, Sitthichai; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
Int. j. morphol ; 36(2): 447-453, jun. 2018. tab, graf
Article in English | LILACS | ID: biblio-954135
ABSTRACT
Although Momordica charantia (MC) has preventive effects on tissue injuries, antioxidant capacity and protective effect of MC pulp and peel (MCP) on valproic acid (VPA)-testicular damage have never been reported. Fresh MCPs were aqueous extracted and determined for antioxidant capacity and momordicine I level by HPLC. Male rats were divided into 5 groups (control, VPA (500 mg/kgBW), MCP20/40/ or 80 mg/kgBW+VPA). In 30 experimental days, animals were pretreated with different doses of MCPs for 20 days before VPA injection for 10 consecutive days. Sperm concentration, testosterone hormone, and testicular histology of all groups were investigated. Expressions of testicular tyrosine phosphorylated and steroidogenic acute regulatory (StAR) proteins were examined by Western blot. Results showed that MCP contains TPC (39.24±0.65 ug/mg garlic acid), antioxidant capacities (FRAP=33.08±0.21 ug/ mg ascorbic acid equivalent, IC50 of DPPH=389.8±3.20 ug/ml), and momordicine I (404.9 mg/g MCP). Sperm concentration in MCP80+VPA group was increased as compared to VPA group. Testosterone level in MCP treated groups was significantly increased. MCP protected testicular damage and could prevent the decrease of StAR and a 50-kDa phosphorylated protein expression in VPAtreated testis. In conclusion, MCP has antioxidant activities and can prevent male reproductive toxicity in VPA-induced rats.
RESUMEN
A pesar que la Momordica charantia (MC) tiene efectos preventivos sobre las lesiones en los tejidos, capacidad antioxidante y un efecto protector de la pulpa y la cáscara de MC (CMC) sobre el ácido valproico (AVP), aún no se ha informado efectos sobre el daño testicular. Las CMC frescas fueron extraídas de forma acuosa y se determinó la capacidad antioxidante y el nivel de Momordicina I por HPLC. Las ratas machos se dividieron en 5 grupos control, AVP (500 mg/kg de peso corporal), CMC20 / 40 / u 80 mg/kg de peso corporal + AVP . En 30 días experimentales, los animales fueron pretratados con diferentes dosis de CMC durante 20 días antes de la inyección de AVP durante 10 días consecutivos. Se investigó la concentración de espermatozoides, la hormona testosterona y la histología testicular de todos los grupos. Las expresiones de proteínas reguladoras agudas (StAR) fosforiladas con tirosina y esteroidogénicas testiculares se examinaron mediante inmunotransferencia de tipo Western. Los resultados mostraron que CMC contiene TPC (39.24 ± 0.65 ug / mg de ácido de ajo), capacidades antioxidantes (FRAP = 33.08 ± 0.21 ug / mg de ácido ascórbico equivalente, IC50 de DPPH = 389.8 ± 3.20 ug / ml) y momordicina I (404.9 mg) / g CMC). La concentración de esperma en el grupo MCP80 + AVP aumentó en comparación con el grupo AVP. El nivel de testosterona en los grupos tratados con CMC aumentó significativamente. La CMC protegió el daño testicular y pudo prevenir la disminución de StAR y una expresión de proteína fosforilada de 50 kDa en los testículos tratados con AVP. En conclusión, la CMC tiene efectos antioxidantes y puede prevenir la toxicidad reproductiva en ratas machos inducidas por VPA.
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Full text: Available Index: LILACS (Americas) Main subject: Testis / Plant Extracts / Momordica charantia / Antioxidants Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article / Project document Affiliation country: Thailand Institution/Affiliation country: Khon Kaen University/TH / Mahasarakham University/TH

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Full text: Available Index: LILACS (Americas) Main subject: Testis / Plant Extracts / Momordica charantia / Antioxidants Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article / Project document Affiliation country: Thailand Institution/Affiliation country: Khon Kaen University/TH / Mahasarakham University/TH