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Localization and changes of tyrosine phosphorylated proteins and ß actin in epididymis of rats treated with valproic acid / Localización y cambios de las proteínas tirosina fosforiladas y la ß actina en epidídimos de ratas tratadas con ácido valproico
Sawatpanich, Tarinee; Arun, Supatcharee; Tongpan, Saranya; Chaichun, Amnart; Sampannang, Apichakarn; Sukhorum, Wannisa; Maneenin, Chanwit; Burawat, Jaturon; Iamsaard, Sitthichai.
  • Sawatpanich, Tarinee; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Arun, Supatcharee; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Tongpan, Saranya; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Chaichun, Amnart; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Sampannang, Apichakarn; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
  • Sukhorum, Wannisa; Mae Fah Luang University. School of Medicine. Chiang Rai. TH
  • Maneenin, Chanwit; Ubonratchathani University. College of Medicine and Public Health. Department of Anatomy. TH
  • Burawat, Jaturon; Ubonratchathani University. College of Medicine and Public Health. Department of Anatomy. TH
  • Iamsaard, Sitthichai; Khon Kaen University. Faculty of Medicine. Department of Anatomy. Khon Kaen. TH
Int. j. morphol ; 36(3): 835-840, Sept. 2018. graf
Article in English | LILACS | ID: biblio-954194
ABSTRACT
Tyrosine phosphorylated proteins have been localized and identified in male reproductive tissues such as testis and capacitated/ acrosome reacted sperm except epididymis. The changes of such proteins are associated with decreased sperm quality of valproic acid treatment. This study aimed to investigate the presence and alterations of protein phosphorylation in epididymal epithelium and fluid of rats treated VPA. Sixteen adult male rats were divided into control and VPA-treated groups (n=8/ each). Treated rats were injected with VPA (500 mg/ kgBW, intraperitoneally) for 10 consecutive days. At the end of experiment, the monoclonal antiphosphotyrosine (clone 4G10) was used for immunohistochemistry to probe tyrosine phosphorylated proteins and also to examine the expression of such proteins using immuno-Western blotting in epididymal tissue and fluid. The result showed that positive reactivity of phosphorylated proteins was clearly observed in cytoplasmic principle cells, nuclei of apical & basal cells and sperm mass surrounded with epididymal fluids. The profiles of phosphorylated proteins in epididymal fluid were 182, 127, 80, 70, 57, 45, 34, and 31 kDas, respectively. Interestingly, VPA affected the changes of phosphorylated proteins and β actin in head, body, and tail epididymal fluids. We conclude that tyrosine phosphorylated proteins were detected in epididymal epithelium and fluid. The expressions of those proteins and actin were altered under VPA treating.
RESUMEN
Las proteínas tirosina fosforiladas han sido localizadas e identificadas en tejidos reproductores masculinos tales como testículos y espermatozoides, capacitados a nivel acrosómico, excepto en el epidídimo. Los cambios de estas proteínas están asociadas con una disminución de la calidad del esperma en el tratamiento con ácido valproico (AVP). Este estudio tuvo como objetivo investigar la presencia y las alteraciones de la fosforilación de proteínas en el epitelio epididimal y en el fluido espermático de ratas tratadas con AVP. Dieciséis ratas macho adultas se dividieron en dos grupos control y tratadas con AVP (n = 8 / cada uno). A las ratas tratadas se les inyectó AVP por vía intraperitoneal (500 mg / kg de peso corporal) durante 10 días consecutivos. Al final del experimento, se realizó inmunohistoquímica con la anti-fosfotirosina monoclonal (clon 4G10) para sondear las proteínas tirosina fosforiladas y también para examinar la expresión de tales proteínas usando inmunotransferencia Western, en tejido y fluido epididimarios. El resultado mostró reactividad positiva de proteínas fosforiladas en células citoplásmicas principales, en los núcleos de las células apicales y basales y en la masa de esperma rodeada por fluidos epididimarios. Los perfiles de proteínas fosforiladas en el fluido epididimal fueron 182, 127, 80, 70, 57, 45, 34 y 31 kDas, respectivamente. El AVP provocó cambios en las proteínas fosforiladas y en la β actina de los fluidos epididimarios de cabeza, cuerpo y cola del epidídimo. Concluimos que las proteínas tirosina fosforiladas se detectaron en el epitelio y el fluido epididimarios. Las expresiones de esas proteínas y de la β actina se alteraron bajo tratamiento con AVP.
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Full text: Available Index: LILACS (Americas) Main subject: Phosphoproteins / Tyrosine / Valproic Acid / Actins / Anticonvulsants Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article / Project document Affiliation country: Thailand Institution/Affiliation country: Khon Kaen University/TH / Mae Fah Luang University/TH / Ubonratchathani University/TH

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Full text: Available Index: LILACS (Americas) Main subject: Phosphoproteins / Tyrosine / Valproic Acid / Actins / Anticonvulsants Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article / Project document Affiliation country: Thailand Institution/Affiliation country: Khon Kaen University/TH / Mae Fah Luang University/TH / Ubonratchathani University/TH