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Metformin protects against thioacetamide induced liver injury in rats / La metformina protege contra la lesión hepática inducida por tioacetamida en ratas
Al-Hashem, Fahaid; AlHumayed, Suliman; Ellatif, Mohamed Abd; Amin, Shaimaa Nasr; Kamar, Samaa Samir; Al-Ani, Bahjat; Haidara, Mohamed A.
  • Al-Hashem, Fahaid; King Khalid University. College of Medicine. Departments of Physiology. Abha. SA
  • AlHumayed, Suliman; King Khalid University. College of Medicine. Departments of Medicine. Abha. SA
  • Ellatif, Mohamed Abd; King Khalid University. College of Medicine. Departments of Biochemistry. Abha. SA
  • Amin, Shaimaa Nasr; Cairo University. Kasr Al-Aini Faculty of Medicine. Departments of Physiology. Cairo. EG
  • Kamar, Samaa Samir; Cairo University. Kasr Al-Aini Faculty of Medicine. Departments of Medical Histology. Cairo. EG
  • Al-Ani, Bahjat; King Khalid University. College of Medicine. Departments of Physiology. Abha. SA
  • Haidara, Mohamed A; King Khalid University. College of Medicine. Departments of Physiology. Abha. SA
Int. j. morphol ; 36(3): 984-990, Sept. 2018. graf
Article in English | LILACS | ID: biblio-954219
ABSTRACT
Potent heptatotoxic chemicals such as carbon tetrachloride and thioacetamide (TAA) are used to evaluate hepatoprotective agents. Here we sought to investigate the potential protective effect of the antidiabetic and antioxidant drug, metformin against liver injury induced by TAA. Model group rats received several injections of TAA (200 mg/kg) before being sacrificed after 10 weeks and the protective group started the treatment two weeks prior to TAA injections and continued receiving both agents, metformin and TAA until the end of the experiment, week 10. Harvested liver tissues were examined using light microscopy and liver homogenates were assayed for oxidative and anti-oxidative stress markers that are known to be modulated in liver injury. Profound damage in the hepatic tissue of the model group such as liver fibrosis and destruction of hepatic architectures were revealed, which were protected by metformin comparable to the control group. TAA augmented the oxidative stress biomarker, malondialdehyde (MDA) and ameliorated the antioxidant superoxide dismutase (SOD), which were significantly (p<0.05) protected by metformin treatment. These results indicate that metformin effectively protects against TAA-induced hepatotoxicity in a rat model.
RESUMEN
Para evaluar los agentes hepatoprotectores se usan químicos heptatotóxicos potentes como el tetracloruro de carbono y la tioacetamida (TAA). En este estudio tratamos de investigar el efecto protector potencial de la droga antidiabética y antioxidante, la metformina contra la lesión hepática inducida por TAA. Las ratas del grupo modelo recibieron varias inyecciones de TAA (200 mg/kg) durante 10 semanas antes de ser sacrificadas, y el grupo protector comenzó el tratamiento dos semanas antes de las inyecciones TAA y continuó recibiendo ambos agentes, metformina y TAA, hasta el final del experimento. Los tejidos hepáticos se examinaron usando microscopía óptica y se analizaron los homogeneizados hepáticos en busca de marcadores de estrés oxidativo y antioxidante los que están modulados en la lesión hepática. Se observaron daños significativos en el tejido hepático del grupo modelo como la fibrosis hepática y destrucción de la arquitectura hepática, que estaban protegidas por la metformina comparable al grupo control. TAA aumentó el biomarcador de estrés oxidativo, malondialdehído (MDA) y mejoró la enzima antioxidante superóxido dismutasa (SOD), que fueron protegidas significativamente (p <0,05) por el tratamiento con metformina. Estos resultados indican que la metformina protege eficazmente contra la hepatotoxicidad inducida por TAA en un modelo de rata.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Thioacetamide / Chemical and Drug Induced Liver Injury / Hypoglycemic Agents / Metformin Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article / Project document Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: Cairo University/EG / King Khalid University/SA

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Full text: Available Index: LILACS (Americas) Main subject: Thioacetamide / Chemical and Drug Induced Liver Injury / Hypoglycemic Agents / Metformin Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article / Project document Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: Cairo University/EG / King Khalid University/SA