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Ghrelin improves the fine structure of atrial natriuretic factor (ANF) granules and intercalated disc junctions in experimentally-induced myocardial infarction in rats / La grelina mejora las estructuras finas de los gránulos de factor natriurético atrial (ANF) y las uniones intercaladas de disco en el infarto de miocardio inducido experimentalmente en ratas
Eid, Refaat A; Zaki, Mohamed Samir Ahmed; Alghamdi, Mansour A; Al-Shraim, Mubarak; El-kott, Attalla Farag; Al-Hashem, Fahaid H; Aldera, Hussain; Alkhateeb, Mahmoud A.
  • Eid, Refaat A; King Khalid University. College of Medicine. Department of Pathology. Abha. SA
  • Zaki, Mohamed Samir Ahmed; King Khalid University. College of Medicine. Department of Anatomy. Abha. SA
  • Alghamdi, Mansour A; King Khalid University. College of Medicine. Department of Anatomy. Abha. SA
  • Al-Shraim, Mubarak; King Khalid University. College of Medicine. Department of Pathology. Abha. SA
  • El-kott, Attalla Farag; King Khalid University. College of Science. Department of Biology. Abha. SA
  • Al-Hashem, Fahaid H; King Khalid University. College of Medicine. Department of Physiology. Abha. SA
  • Aldera, Hussain; King Saud bin Abdulaziz University for Health Sciences. College of Medicine. Department of basic medical Sciences. SA
  • Alkhateeb, Mahmoud A; King Saud bin Abdulaziz University for Health Sciences. College of Medicine. Department of basic medical Sciences. SA
Int. j. morphol ; 36(3): 1031-1042, Sept. 2018. tab, graf
Article in English | LILACS | ID: biblio-954227
Responsible library: CL1.1
ABSTRACT
Ghrelin is a novel growth hormone-releasing peptide administered to treat myocardial infarction (MI). However, the underlying mechanism of its protective effects against MI remains unclear. A total of sixty healthy Sprague Dawley male rats were included. The first one is the sham-operated control group were the rats that underwent the same surgical used to induce MI but without tying the left anterior descending coronary artery (LAD) and received normal saline (0.5 ml) as vehicle; the second MI model group were rats with LAD ligation and received normal saline (0. 5 ml) and the third one is MI+ghrelin group were rats that were exposed to surgery to induce MI but received ghrelin (100 µ/kg, orally, 2x/day). At the end of the experiment after 21 days post-MI, rats were sacrificed and processed for ultrastructural demonstration. Our experiment showed that ghrelin inhibited cardiomyocyte apoptosis. Concomitant administration of ghrelin with MI treated rats of this study appeared to show a considerable protection of the atrial tissues. This study revealed that the sarcoplasm was occupied by normal myofibrils with clear striations and others appeared with minor disruption. Normal distribution of atrionatriuretic factor (ANF) granules and well preserved mitochondrial integrity (preserved cristae, normal size and shape), nucleus chromatin arrangement and striated pattern of clear bands (Z and H) compared to the MI group. Intact intercalated disc with clear identification of fully formed fascia adherence and desmosomes with a reconstruction of gap junction (nexus) was also noticed. Atrial myocytes after myocardial infarction is often associated with subsequent heart failure, which could lead to a fatal outcome. In a rat model of experimental myocardial infarction, peripheral ghrelin administration attenuated myocyte dysfunction, well-preserved desmosome, adherent and gap junction of the intercalated disc and normally distributed ANF granules.
RESUMEN
La grelina es un nuevo péptido liberador de hormona de crecimiento administrado para tratar el infarto de miocardio (IM). Sin embargo, el mecanismo subyacente de sus efectos protectores contra el IM aún no se conocen. Se incluyeron un total de 60 ratas macho Sprague Dawley saludables. En el grupo control se incluyeron ratas que fueron sometidas a una cirugía utilizada para inducir el IM, pero sin ligar la arteria coronaria descendente anterior izquierda (ACDAI) y recibieron suero fisiológico normal (0,5 ml) como vehículo; el segundo grupo modelo de IM fueron ratas con ligadura de ACDAI y recibieron suero fisiológico normal (0,5 ml); el tercer grupo estuvo formado por ratas con IM + grelina, expuestas a la cirugía para inducir IM pero luego recibieron grelina (100 m/kg, oralmente, 2x/día). Al final del experimento, 21 días después del infarto de miocardio, los animales fueron sacrificados y procesados para el estudio ultraestructural. Nuestro experimento mostró que la grelina inhibe la apoptosis de los cardiomiocitos. La administración concomitante de grelina en ratas con IM parece indicar una protección considerable de los tejidos atriales. Además, el estudio reveló que el sarcoplasma estaba ocupado por miofibrillas normales con estriaciones claras y otras con una alteración menor. Se encontró una distribución normal de los gránulos del factor natriurético atrial (FNA) e integridad mitocondrial bien conservada (crestas conservadas, tamaño y forma normales), disposición de la cromatina del núcleo y patrón estriado de bandas claras (Z y H) en comparación con el grupo IM. También se observó un disco intercalado intacto con una clara identificación de la adherencia de la fascia completamente formada y desmosomas con una reconstrucción de la unión gap (nexo). Los miocitos atriales, después de un infarto de miocardio, a menudo se asocian con insuficiencia cardíaca posterior, que podría conducir a un desenlace fatal. En un modelo de rata de infarto de miocardio experimental, la administración de grelina periférica atenuó la disfunción de miocitos, con conservación del desmosoma, adherencia y unión de la brecha del disco intercalado y una distribución normal de los los gránulos de FNA.
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Full text: Available Index: LILACS (Americas) Main subject: Atrial Natriuretic Factor / Peptide Hormones / Myocardial Infarction Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article / Project document Affiliation country: Saudi Arabia Institution/Affiliation country: King Khalid University/SA / King Saud bin Abdulaziz University for Health Sciences/SA

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Full text: Available Index: LILACS (Americas) Main subject: Atrial Natriuretic Factor / Peptide Hormones / Myocardial Infarction Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article / Project document Affiliation country: Saudi Arabia Institution/Affiliation country: King Khalid University/SA / King Saud bin Abdulaziz University for Health Sciences/SA