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Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium / Expansión y cambios fenotípicos de células mesenquimales de médula ósea de ratón cultivadas con FGF-2 y medio condicionado por el nervio facial
Lucena, Eudes Euler de Souza; de-Morais, Hécio Henrique Araújo; Araújo, Dayane Pessoa de; Cavalcanti, José Rodolfo Lopes de Paiva; Azevedo, Eduardo Pereira de; Queiroz, Dinalva Brito de; Botelho, Marco Antônio; Rêgo, Amália Cinthia Meneses do; Filho, Irami Araújo; Barboza, Carlos Augusto Galvão; Nascimento Júnior, Expedito Silva do; Costa, Miriam Stela Maris de Oliveira; Cavalcante, Jeferson de Sousa; Guzen, Fausto Pierdoná.
  • Lucena, Eudes Euler de Souza; State University of Rio Grande do Norte. Health Science Center. Department of Biomedical Sciences. Mossoró. BR
  • de-Morais, Hécio Henrique Araújo; State University of Rio Grande do Norte. Health Science Center. Department of Biomedical Sciences. Mossoró. BR
  • Araújo, Dayane Pessoa de; State University of Rio Grande do Norte. Health Science Center. Department of Biomedical Sciences. Mossoró. BR
  • Cavalcanti, José Rodolfo Lopes de Paiva; State University of Rio Grande do Norte. Health Science Center. Department of Biomedical Sciences. Mossoró. BR
  • Azevedo, Eduardo Pereira de; Potiguar University. Health School. Natal. BR
  • Queiroz, Dinalva Brito de; Potiguar University. Health School. Natal. BR
  • Botelho, Marco Antônio; Potiguar University. Health School. Natal. BR
  • Rêgo, Amália Cinthia Meneses do; Potiguar University. Health School. Natal. BR
  • Filho, Irami Araújo; State University of Rio Grande do Norte. Health Science Center. Department of Biomedical Sciences. Mossoró. BR
  • Barboza, Carlos Augusto Galvão; Federal University of Rio Grande do Norte. Bioscience Center. Department of Morphology. Natal. BR
  • Nascimento Júnior, Expedito Silva do; Federal University of Rio Grande do Norte. Bioscience Center. Department of Morphology. Natal. BR
  • Costa, Miriam Stela Maris de Oliveira; Federal University of Rio Grande do Norte. Bioscience Center. Department of Morphology. Natal. BR
  • Cavalcante, Jeferson de Sousa; Federal University of Rio Grande do Norte. Bioscience Center. Department of Physiology. Natal. BR
  • Guzen, Fausto Pierdoná; State University of Rio Grande do Norte. Health Science Center. Department of Biomedical Sciences. Mossoró. BR
Int. j. morphol ; 36(3): 1049-1056, Sept. 2018. graf
Article in English | LILACS | ID: biblio-954229
ABSTRACT
Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation.
RESUMEN
Las células mesenquimales (CM) exhiben un gran potencial regenerativo debido a sus propiedades intrínsecas y la capacidad de restaurar la función del tejido, ya sea directamente, a través de la transdiferenciación, o indirectamente, a través de efectos parácrinos. Este estudio tuvo como objetivo evaluar los cambios morfométricos y fenotípicos en CM cultivadas con medio condicionado por nervio facial en presencia o ausencia de factor de crecimiento de fibroblastos 2 (FGF-2). Para el análisis fenotípico cuantitativo, se analizó la expresión de GFAP, OX-42, MAP-2, β-tubulina III, NeuN y NF-200 mediante inmunocitoquímica. Las células cultivadas con medio condicionado por el nervio facial en presencia de FGF-2 expresaban GFAP, OX-42, MAP-2, β-tubulina III, NeuN y NF-200. En promedio, el área y el perímetro de las células positivas para GFAP fueron mayores en el grupo cultivado con medio condicionado por el nervio facial en comparación con el grupo cultivado con medio acondicionado y FGF-2 (p = 0,0001). Este estudio demostró la plasticidad de CM para linajes neuronales y gliales y abre nuevas perspectivas de investigación en terapia celular y transdiferenciación.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Bone Marrow / Fibroblast Growth Factor 2 / Facial Nerve Injuries / Mesenchymal Stem Cells Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Rio Grande do Norte/BR / Potiguar University/BR / State University of Rio Grande do Norte/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bone Marrow / Fibroblast Growth Factor 2 / Facial Nerve Injuries / Mesenchymal Stem Cells Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Rio Grande do Norte/BR / Potiguar University/BR / State University of Rio Grande do Norte/BR