Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice
J. appl. oral sci
;
26: e20170484, 2018. tab, graf
Article
in English
| LILACS, BBO
| ID: biblio-954527
ABSTRACT
Abstract Objective This study aimed to demonstrate the immunohistochemical changes associated with MMP-2 and type 1 collagen separately for the first time in the major salivary glands (the parotid, submaxillary, and sublingual glands) that occur with aging in mice. Material and Methods Fourteen Balb/c white mice (50-80 g) were used in this study. The animals were divided into two equal groups. Group I consisted of young animals (2-month-old) (n=7) and Group II consisted of older animals (18-month-old) (n=7). After routine histological follow-ups, Hematoxylin-eosin (H&E), Masson's Trichrome staining and immunohistochemical staining was performed for type I collagen and MMP-2. Results We observed that there were age-related decreases in the number of acinar cells, increase in eosinophilic zymogen granules in cells, collagen accumulation in fibrotic areas and dilatation in interlobular ducts. Also, while type I collagen and MMP-2 immunoreactivity were moderate in the salivary glands of the young mice, they were high in the salivary glands of the old mice (p=0.001). In the H-score assessment, MMP-2 immunoreactivity was lower at a significant level in young mice than in old mice (p=0.001). Conclusions This study showed that anatomical, physiological and morphological abnormalities occur in all three major salivary glands as a natural consequence of aging.
Full text:
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Index:
LILACS (Americas)
Main subject:
Parotid Gland
/
Sublingual Gland
/
Submandibular Gland
/
Matrix Metalloproteinase 2
/
Collagen Type I
Limits:
Animals
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2018
Type:
Article
Affiliation country:
Turkey
Institution/Affiliation country:
Gaziomanspasa University/TR
/
Kahramanmaras Sütcü Imam University/TR
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