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Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders
Arán-Sekul, Tomás; Rojas, José M; Subiabre, Mario; Cruz, Victoria; Cortés, William; Osorio, Luis; González, Jorge; Araya, Jorge E; Catalán, Alejandro.
  • Arán-Sekul, Tomás; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
  • Rojas, José M; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
  • Subiabre, Mario; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
  • Cruz, Victoria; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
  • Cortés, William; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
  • Osorio, Luis; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
  • González, Jorge; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
  • Araya, Jorge E; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
  • Catalán, Alejandro; University of Antofagasta. Faculty of Health Sciences. Department of Medical Technology. Antofagasta. CL
Article in English | LILACS | ID: biblio-954860
ABSTRACT
Loxoscelism is a severe human envenomation caused by Loxosceles spider venom. To the best of our knowledge, no study has evaluated the presence of antibodies against Loxosceles venom in loxoscelism patients without treatment with antivenom immunotherapy. We perform a comparative analysis for the presence of antibodies capable of recognizing Loxosceles venom in a group of patients diagnosed with loxoscelism and in a group of people without loxoscelism. Methods The detection of L. laeta venom, Sicarius venom and recombinant phospholipases D from Loxosceles (PLDs) in sera from people with loxoscelism (Group 1) and from healthy people with no history of loxoscelism (Group 2) was evaluated using immuno-dot blot, indirect ELISA, and Western blot. Results We found naturally heterophilic antibodies (IgG-type) in people without contact with Loxosceles spiders or any clinical history of loxoscelism. Either serum pools or single sera from Group 1 and Group 2 analyzed by dot blot tested positive for L. laeta venom. Indirect ELISA for venom recognition showed titles of 1320 for Group 1 sera and 1160 for Group 2 sera. Total IgG quantification showed no difference in sera from both groups. Pooled sera and purified IgG from sera of both groups revealed venom proteins between 25 and 32 kDa and the recombinant phospholipase D isoform 1 (rLlPLD1), specifically. Moreover, heterophile antibodies cross-react with PLDs from other Loxosceles species and the venom of Sicarius spider. Conclusions People without contact with the spider venom produced heterophilic antibodies capable of generating a cross-reaction against the venom of L. laeta and Sicarius spiders. Their presence and possible interference should be considered in the development of immunoassays for Loxosceles venom detection.(AU)
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Full text: Available Index: LILACS (Americas) Main subject: Phospholipase D / Spider Venoms / Antibodies, Heterophile / Antivenins Limits: Adolescent / Adult / Female / Humans / Male Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2018 Type: Article / Project document Affiliation country: Chile Institution/Affiliation country: University of Antofagasta/CL

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Full text: Available Index: LILACS (Americas) Main subject: Phospholipase D / Spider Venoms / Antibodies, Heterophile / Antivenins Limits: Adolescent / Adult / Female / Humans / Male Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2018 Type: Article / Project document Affiliation country: Chile Institution/Affiliation country: University of Antofagasta/CL