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Structural insights into Plasmodium falciparum nicotinamide mononucleotide adenylyltransferase: oligomeric assembly
Contreras-Rodríguez, Luis Ernesto; Marin-Mogollon, Catherin Yizet; Sánchez-Mejía, Lina Marcela; Ramírez-Hernández, María Helena.
  • Contreras-Rodríguez, Luis Ernesto; Universidad Nacional de Colombia. Facultad de Ciencias. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Marin-Mogollon, Catherin Yizet; Universidad Nacional de Colombia. Facultad de Ciencias. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Sánchez-Mejía, Lina Marcela; Universidad Nacional de Colombia. Facultad de Ciencias. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Ramírez-Hernández, María Helena; s.af
Mem. Inst. Oswaldo Cruz ; 113(9): e180073, 2018. tab, graf
Article in English | LILACS | ID: biblio-955126
ABSTRACT
The biochemical pathways involved in nicotinamide adenine dinucleotide (NAD) biosynthesis converge at the enzymatic step catalysed by nicotinamide mononucleotide adenylyltransferase (NMNAT, EC 2.7.7.1). The majority of NMNATs are assembled into homo-oligomeric states that comprise 2-6 subunits. Recently, the NMNAT of Plasmodium falciparum (PfNMNAT) has been identified as a pharmacological target. The enzymatic characterisation, cellular location, and tertiary structure of the PfNMNAT protein have been reported. Nonetheless, its quaternary structure remains to be explored. The present study describes the oligomeric assembly of the 6 x His-PfNMNAT recombinant protein using immobilised metal affinity chromatography coupled with size exclusion chromatography (SEC) and native protein electrophoresis combined with Ferguson plot graphing. These chromatographic approaches resulted in the elution of an active monomer from the SEC column, whereas the Ferguson plot indicated a dimeric assembly of the 6 x His-PfNMNAT protein.
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Full text: Available Index: LILACS (Americas) Main subject: Plasmodium falciparum Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2018 Type: Article Affiliation country: Colombia Institution/Affiliation country: Universidad Nacional de Colombia/CO

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Full text: Available Index: LILACS (Americas) Main subject: Plasmodium falciparum Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2018 Type: Article Affiliation country: Colombia Institution/Affiliation country: Universidad Nacional de Colombia/CO