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Quantitative determination of monotropein in rat plasma and tissue by LC-MS/MS and its application to pharmacokinetic and tissue distribution studies
Zhou, Qiang; Yan, He; Li, Rui; Li, Xingliang; Wei, Jingyan.
  • Zhou, Qiang; Jilin University. College of Pharmaceutical Science. Changchun. CN
  • Yan, He; The First Hospital of Jilin University. Department of Emergency. Changchun. CN
  • Li, Rui; University of Chinese Medicine. The Affiliated Hospital to Changchun. Changchun. CN
  • Li, Xingliang; The First Hospital of Jilin University. Department of Emergency. Changchun. CN
  • Wei, Jingyan; Jilin University. College of Pharmaceutical Science. Changchun. CN
Rev. bras. farmacogn ; 28(4): 451-456, July-Aug. 2018. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-958884
Responsible library: BR1.1
ABSTRACT
Abstract A selective and sensitive liquid chromatography tandem with mass spectrometry was developed and validated for accurate determination of monotropein in rat plasma and tissues. All biological samples were prepared by simple protein precipitation method using catalpol as an internal standard. The analyte and internal standard were separated on a C18 analytical column with 2 min of run time, at flow rate of 0.5 ml/min. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped with negative-ion electrospray ionization by selected-reaction monitoring of the transitions at m/z 389→147 for monotropein and m/z 361→169 for the internal standard. The calibration curves for plasma and tissue samples were linear over the concentration range of 4-2000 ng/ml, with a lower limit of quantification of 4 ng/ml. The method was successfully applied to a pharmacokinetic and tissue distribution study of monotropein in rats.


Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. bras. farmacogn Journal subject: Pharmacy Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Jilin University/CN / The First Hospital of Jilin University/CN / University of Chinese Medicine/CN

Full text: Available Index: LILACS (Americas) Language: English Journal: Rev. bras. farmacogn Journal subject: Pharmacy Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Jilin University/CN / The First Hospital of Jilin University/CN / University of Chinese Medicine/CN