The role of the exogenous supply of adenosine triphosphate in the expression of Bax and Bcl2L1 genes in intestinal ischemia and reperfusion in rats
Acta cir. bras
;
33(10): 889-895, Oct. 2018. tab
Article
in English
| LILACS
| ID: biblio-973469
ABSTRACT
Abstract Purpose:
To investigate the role of the exogenous supply of adenosine triphosphate (ATP) in the expression of Bax and Bcl2L1 genes in intestinal ischemia and reperfusion (IR) in rats.Methods:
The study was designed as a randomized controlled trial with a blinded assessment of the outcome. Eighteen adult male Wistar-EPM1 rats were housed under controlled temperature and light conditions (22-23°C, 12 h light/dark cycle). The animals were randomly divided into 3 groups 1. Sham group (SG) no clamping of the superior mesenteric artery; 2. Ischemia and reperfusion group (IRG) 3. Ischemia and reperfusion plus ATP (IRG + ATP). ATP was injected in the femoral vein before and after ischemia. Afterwards, intestinal segments were appropriately removed and processed for Endothelial Cell Biology Rat RT2 Profiler PCR Array.Results:
ATP promoted the upregulation of Bcl2L1 gene expression, whereas it did not have significant effects on Bax gene expression. In addition, the relation of Bax/Bcl2L1 gene expression in the IRG group was 1.39, whereas it was 0.43 in the IRG + ATP group. Bcl2L1 plays a crucial role in protecting against intestinal apoptosis after ischemia and reperfusion. Increased Bcl2L1 expression can inhibit apoptosis while decreased Bcl2L1 expression can trigger apoptosis.Conclusion:
Adenosine triphosphate was associated with antiapoptotic effects on the rat intestine ischemia and reperfusion by upregulating of Bcl2L1 gene expression.
Full text:
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Index:
LILACS (Americas)
Main subject:
Adenosine Triphosphate
/
Apoptosis
/
Genes, bcl-2
/
Bcl-2-Associated X Protein
/
Ischemia
Type of study:
Etiology study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
UNIFESP/BR
/
Universidade Anhembi Morumbi/BR
/
Universidade Federal de São Paulo/BR
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