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The role of the exogenous supply of adenosine triphosphate in the expression of Bax and Bcl2L1 genes in intestinal ischemia and reperfusion in rats
Fagundes, Djalma José; Carrara, Fernando Lopes; Teixeira, William Andrade; Simões, Ricardo Santos; Taha, Murched Omar.
  • Fagundes, Djalma José; Universidade Federal de São Paulo. Department of Surgery. Division of Surgical Techniques and Experimental Surgery. Sao Paulo. BR
  • Carrara, Fernando Lopes; Universidade Anhembi Morumbi. Sao Paulo. BR
  • Teixeira, William Andrade; Universidade Anhembi Morumbi. Sao Paulo. BR
  • Simões, Ricardo Santos; UNIFESP. Department of Morphology and Genetic. Sao Paulo. BR
  • Taha, Murched Omar; UNIFESP. Department of Surgery. Division of Surgical Techniques and Experimental Surgery. Sao Paulo. BR
Acta cir. bras ; 33(10): 889-895, Oct. 2018. tab
Article in English | LILACS | ID: biblio-973469
ABSTRACT
Abstract

Purpose:

To investigate the role of the exogenous supply of adenosine triphosphate (ATP) in the expression of Bax and Bcl2L1 genes in intestinal ischemia and reperfusion (IR) in rats.

Methods:

The study was designed as a randomized controlled trial with a blinded assessment of the outcome. Eighteen adult male Wistar-EPM1 rats were housed under controlled temperature and light conditions (22-23°C, 12 h light/dark cycle). The animals were randomly divided into 3 groups 1. Sham group (SG) no clamping of the superior mesenteric artery; 2. Ischemia and reperfusion group (IRG) 3. Ischemia and reperfusion plus ATP (IRG + ATP). ATP was injected in the femoral vein before and after ischemia. Afterwards, intestinal segments were appropriately removed and processed for Endothelial Cell Biology Rat RT2 Profiler PCR Array.

Results:

ATP promoted the upregulation of Bcl2L1 gene expression, whereas it did not have significant effects on Bax gene expression. In addition, the relation of Bax/Bcl2L1 gene expression in the IRG group was 1.39, whereas it was 0.43 in the IRG + ATP group. Bcl2L1 plays a crucial role in protecting against intestinal apoptosis after ischemia and reperfusion. Increased Bcl2L1 expression can inhibit apoptosis while decreased Bcl2L1 expression can trigger apoptosis.

Conclusion:

Adenosine triphosphate was associated with antiapoptotic effects on the rat intestine ischemia and reperfusion by upregulating of Bcl2L1 gene expression.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Adenosine Triphosphate / Apoptosis / Genes, bcl-2 / Bcl-2-Associated X Protein / Ischemia Type of study: Etiology study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: UNIFESP/BR / Universidade Anhembi Morumbi/BR / Universidade Federal de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Adenosine Triphosphate / Apoptosis / Genes, bcl-2 / Bcl-2-Associated X Protein / Ischemia Type of study: Etiology study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: UNIFESP/BR / Universidade Anhembi Morumbi/BR / Universidade Federal de São Paulo/BR