Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice
Acta cir. bras
;
33(11): 991-999, Nov. 2018. graf
Article
in English
| LILACS
| ID: biblio-973476
ABSTRACT
Abstract Purpose:
To determine whether the absence of transglutaminase 2 enzyme (TG2) in TG2 knockout mice (TG2-/-) protect them against early age-related functional and histological arterial changes.Methods:
Pulse wave velocity (PWV) was measured using non-invasive Doppler and mean arterial pressure (MAP) was measured in awake mice using tail-cuff system. Thoracic aortas were excised for evaluation of endothelial dependent vasodilation (EDV) by wire myography, as well as histological analyses.Results:
PWV and MAP were similar in TG2-/-mice to age-matched wild type (WT) control mice. Old WT mice exhibited a markedly attenuated EDV as compared to young WT animals. The TG2-/-young and old mice had enhanced EDV responses (p<0.01) as compared to WT mice. There was a significant increase in TG2 crosslinks by IHC in WT old group compared to Young, with no stain in the TG2-/-animals. Optical microscopy examination of Old WT mice aorta showed thinning and fragmentation of elastic laminae. Young WT mice, old and young TG2-/-mice presented regularly arranged and parallel elastic laminae of the tunica media.Conclusion:
The genetic suppression of TG2 delays the age-induced endothelial dysfunction and histological modifications.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Aorta, Thoracic
/
Aging
/
Endothelium, Vascular
/
Transglutaminases
/
GTP-Binding Proteins
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2018
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
JHU/US
/
Johns Hopkins University/US
/
UNIFESP/BR
/
UNIVASF/BR
/
Universidade Federal de São Paulo/BR
/
Universidade Federal do Vale do São Francisco/BR
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