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Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window
Monteiro, Betânia Souza; Santos, Bianka Souza dos; Almeida, Bruna Lopes de; Hiura, Emy; Fiorio, Wagner Alexey Back; Valdetaro, Gisele Pereira; Gonçalves, Dariele Vieira; Silva, Caroline Saraiva; Champion, Tatiana; Campagnol, Daniela.
  • Monteiro, Betânia Souza; Universidade Vila Velha. Animal Science Program. Stem Cell and Cellular Therapy Laboratory. BR
  • Santos, Bianka Souza dos; UVV. Animal Science Program. Stem Cell and Cellular Therapy Laboratory. Vila Velha. BR
  • Almeida, Bruna Lopes de; UVV. Animal Science Program. Stem Cell and Cellular Therapy Laboratory. Vila Velha. BR
  • Hiura, Emy; UVV. Animal Science Program. Stem Cell and Cellular Therapy Laboratory. Vila Velha. BR
  • Fiorio, Wagner Alexey Back; Advanced Imaging Center and Veterinary Pathology. Vila Velha. BR
  • Valdetaro, Gisele Pereira; UVV. Animal Science Program. Stem Cell and Cellular Therapy Laboratory. Vila Velha. BR
  • Gonçalves, Dariele Vieira; UVV. Animal Science Program. Stem Cell and Cellular Therapy Laboratory. Vila Velha. BR
  • Silva, Caroline Saraiva; PetScan Diagnoses Center. Vila Velha. BR
  • Champion, Tatiana; Universidade Federal da Fronteira Sul. Veterinary Department. Realeza. BR
  • Campagnol, Daniela; Universidade Federal do Rio Grande do Sul. Clinical Hospital. Animal Experimentation Division. Porto Alegre. BR
Acta cir. bras ; 33(11): 1016-1026, Nov. 2018. graf
Article in English | LILACS | ID: biblio-973481
ABSTRACT
Abstract

Purpose:

To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cell

Methods:

16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphate-buffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal). After the treatments were performed, at 8 and 31 days, four animals in each group were subjected to left nephrectomy for histological studies.

Results:

Histologically, a higher amount of cell debris and tubules devoid of the epithelium and a higher degree of necrosis were observed in the groups treated with PBS, as opposed to a low degree of necrosis and higher tubular vascularization in the groups treated with ADSC, particularly in the group treated with intrarenal ADSC 48 hours after injury.

Conclusion:

The transplantation of ADSC positively contributed to the replacement of necrotic tissue by renal tubular cells, vascularization of the renal parenchyma, and restoration of the organ function.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Adipose Tissue / Acute Kidney Injury / Kidney Type of study: Evaluation studies Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Advanced Imaging Center and Veterinary Pathology/BR / PetScan Diagnoses Center/BR / UVV/BR / Universidade Federal da Fronteira Sul/BR / Universidade Federal do Rio Grande do Sul/BR / Universidade Vila Velha/BR

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Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Adipose Tissue / Acute Kidney Injury / Kidney Type of study: Evaluation studies Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Advanced Imaging Center and Veterinary Pathology/BR / PetScan Diagnoses Center/BR / UVV/BR / Universidade Federal da Fronteira Sul/BR / Universidade Federal do Rio Grande do Sul/BR / Universidade Vila Velha/BR