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Alprostadil attenuates myocardial ischemia/reperfusion injury by promoting antioxidant activity and eNOS activation in rats
Zhang, Liping; Zhang, Ying; Yu, Xiaofeng; Xu, Huali; Sui, Dayuan; Zhao, Xuezhong.
  • Zhang, Liping; Jilin University. First Hospital. Department of Cardiovascular Medicine. Jilin. CN
  • Zhang, Ying; Jilin University. College of Pharmacy. Changchun. CN
  • Yu, Xiaofeng; Jilin University. College of Pharmacy. Changchun. CN
  • Xu, Huali; Jilin University. College of Pharmacy. Changchun. CN
  • Sui, Dayuan; Jilin University,. College of Pharmacy. Changchun. CN
  • Zhao, Xuezhong; Jilin University. First Hospital. Department of Cardiovascular Medicine. Changchun. CN
Acta cir. bras ; 33(12): 1067-1077, Dec. 2018. graf
Article in English | LILACS | ID: biblio-973486
ABSTRACT
Abstract

Purpose:

To investigate the effect of alprostadil on myocardial ischemia/reperfusion (I/R) in rats.

Methods:

Rats were subjected to myocardial ischemia for 30 min followed by 24h reperfusion. Alprostadil (4 or 8 μg/kg) was intravenously administered at the time of reperfusion and myocardial infarct size, levels of troponin T, and the activity of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) in the serum were measured. Antioxidative parameters, nitric oxide (NO) content and phosphorylated endothelial nitric oxide synthase 3 (p-eNOS) expression in the left ventricles were also measured. Histopathological examinations of the left ventricles were also performed.

Results:

Alprostadil treatment significantly reduced myocardial infarct size, serum troponin T levels, and CK-MB and LDH activity (P<0.05). Furthermore, treatment with alprostadil significantly decreased malondialdehyde (MDA) content (P<0.05) and markedly reduced myonecrosis, edema and infiltration of inflammatory cells. Superoxide dismutase and catalase activities (P<0.05), NO level (P<0.01) and p-eNOS (P<0.05) were significantly increased in rats treated with alprostadil compared with control rats.

Conclusion:

These results indicate that alprostadil protects against myocardial I/R injury and that these protective effects are achieved, at least in part, via the promotion of antioxidant activity and activation of eNOS.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Alprostadil / Myocardial Reperfusion Injury / Nitric Oxide Synthase Type III / Antioxidants Type of study: Evaluation studies Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Jilin University/CN / Jilin University,/CN

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Full text: Available Index: LILACS (Americas) Main subject: Alprostadil / Myocardial Reperfusion Injury / Nitric Oxide Synthase Type III / Antioxidants Type of study: Evaluation studies Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: China Institution/Affiliation country: Jilin University/CN / Jilin University,/CN