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Influence of low-level laser irradiation on osteocalcin protein and gene expression in bone tissue1
Bomfim, Fernando Russo Costa do; Sella, Valeria Regina Gonzalez; Thomasini, Ronaldo Luís; Plapler, Helio.
  • Bomfim, Fernando Russo Costa do; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Science. BR
  • Sella, Valeria Regina Gonzalez; UNIFESP. Department of Surgery. Operative Technique and Experimental Surgery Division. Sao Paulo. BR
  • Thomasini, Ronaldo Luís; Universidade Federal dos Vales do Jequitinhonha e Mucuri. Medicine Faculty. Diamantina. BR
  • Plapler, Helio; UNIFESP. Department of Surgery. Operative Technique and Experimental Surgery Division. Sao Paulo. BR
Acta cir. bras ; 33(9): 736-743, Sept. 2018. tab, graf
Article in English | LILACS | ID: biblio-973502
ABSTRACT
Abstract

Purpose:

To evaluate osteocalcin gene and protein expression in vitro and in an in vivo model of ostectomy.

Methods:

Twenty Wistar rats were assigned into two groups A (n=10, laser) and B (n=10, control). Ostectomy was performed in the femur diaphysis; the twenty fragments removed, composed in vitro groups named as in vivo (A and B) and cultivated in CO2 atmosphere for thirteen days. Low-level laser irradiation was performed in groups A (in vivo and in vitro) by an GaAlAs device (λ=808 nm, dose of 2J/cm2, power of 200mW, power density of 0.2W/cm2, total energy of 1.25J, spot diameter of 0.02mm) for 5 seconds, at one point, daily. It was performed immunocytochemistry assays in vivo and in vitro groups. In vitro groups were also submitted to RNA extraction, cDNA synthesis and gene expression by quantitative PCR. Statistical analysis was realized with p<0.05.

Results:

Immunocytochemistry scores showed no significant differences between control and laser groups either in vivo and in vitro. Gene expression also showed no statistical differences.

Conclusion:

Low-level laser irradiation did not alter osteocalcin protein and gene expression in vivo and in vitro in the studied period but it may have been expressed them in an earlier period.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Gene Expression / Osteocalcin / Femur Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: UNIFESP/BR / Universidade Federal de São Paulo/BR / Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR

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Full text: Available Index: LILACS (Americas) Main subject: Gene Expression / Osteocalcin / Femur Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: UNIFESP/BR / Universidade Federal de São Paulo/BR / Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR