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Laboratory evidence for the hematopoietic potential of Beta vulgaris leaf and stalk extract in a phenylhydrazine model of anemia
Gheith, I; El-Mahmoudy, A.
  • Gheith, I; Taibah University. Faculty of Applied Medical Sciences. Department of Clinical Laboratory Sciences. SA
  • El-Mahmoudy, A; Benha University Faculty of Veterinary Medicine. Department of Pharmacology. Moshtohor. EG
Braz. j. med. biol. res ; 51(11): e7722, 2018. tab, graf
Article in English | LILACS | ID: biblio-974246
ABSTRACT
This study was designed to provide laboratory evidence supporting the hematopoietic effect of Beta vulgaris (beet) leaf aqueous extract in phenylhydrazine-induced anemia model in albino rats. Extraction of the leaves/stalks was done by maceration in 30% hydro-ethanol for 48 h. An intraperitoneal injection of 20 mg/kg phenylhydrazine was applied for two consecutive days to develop hemolytic anemia on the 4th day after the 1st injection in 24 of 30 male albino rats. The animals were divided into 5 groups and received the following treatments standard (ferrous ascorbate + folic acid; 13.5 + 0.135 mg/kg), B. vulgaris extract (100 and 200 mg/kg), or left untreated (normal and diseased controls). Blood samples were taken at 0, 4, 8, and 12 days of the experiment for hematological and clinico-chemical analysis. Beet leaf extract significantly restored the levels of red blood cells, white blood cells, hemoglobin, and hematocrit in dose- and time-dependent manners. Blood indices have been significantly corrected. Erythropoietin level was maintained at higher levels. Erythrocytic membrane oxidation biomarker (malondialdehyde) level was significantly reduced compared to the anemic untreated group. The extract exhibited potent, concentration (4-512 μg/mL)-dependent antioxidant activity indicated by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, with IC50 value of 37.91 μg/mL. Beet leaf extract resulted in detection of flavonoid and phenolic compounds that may underlie its hematinic properties. These findings may indicate B. vulgaris as a good natural source for pharmaceutical preparations with hematopoietic effects and treatment of anemia and/or associated conditions.
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Full text: Available Index: LILACS (Americas) Main subject: Plant Extracts / Plant Leaves / Beta vulgaris / Hematinics / Anemia Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2018 Type: Article Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: Benha University Faculty of Veterinary Medicine/EG / Taibah University/SA

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Full text: Available Index: LILACS (Americas) Main subject: Plant Extracts / Plant Leaves / Beta vulgaris / Hematinics / Anemia Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2018 Type: Article Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: Benha University Faculty of Veterinary Medicine/EG / Taibah University/SA