Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
Braz. j. microbiol
;
49(4): 909-913, Oct.-Dec. 2018. tab
Article
in English
| LILACS
| ID: biblio-974301
ABSTRACT
ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40-50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tuberculosis, Pulmonary
/
Polymerase Chain Reaction
/
Real-Time Polymerase Chain Reaction
/
Microscopy
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
Limits:
Adult
/
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2018
Type:
Article
Affiliation country:
Pakistan
Institution/Affiliation country:
Capital University of Science & Technology/PK
/
Khyber Medical University Peshawar/PK
/
National University of Medical Sciences/PK
/
Provincial TB Reference Laboratory/PK
/
Sarhad University of Science and Information Technology/PK
/
University of Haripur/PK
/
University of Peshawar/PK
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