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Performance of "RESIST-3 O.K.N. K-SeT" immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey
Sagiroglu, Pinar; Hasdemir, Ufuk; Gelmez, Gülen Altinkanat; Aksu, Burak; Karatuna, Onur; Söyletir, Güner.
  • Sagiroglu, Pinar; Marmara University. School of Medicine. Department of Medical Microbiology. Istanbul. TR
  • Hasdemir, Ufuk; Marmara University. School of Medicine. Department of Medical Microbiology. Istanbul. TR
  • Gelmez, Gülen Altinkanat; Marmara University. School of Medicine. Department of Medical Microbiology. Istanbul. TR
  • Aksu, Burak; Marmara University. School of Medicine. Department of Medical Microbiology. Istanbul. TR
  • Karatuna, Onur; Acibadem University ,. School of Medicine. Department of Medical Microbiology. Istanbul. TR
  • Söyletir, Güner; Marmara University. School of Medicine. Department of Medical Microbiology. Istanbul. TR
Braz. j. microbiol ; 49(4): 885-890, Oct.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-974312
ABSTRACT
ABSTRACT In this study, the performance of the "RESIST-3 O.K.N. K-SeT" (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla KPC, bla IMP, bla VIM, bla NDM, and bla OXA-48. The rates of bla NDM, bla OXA-48, and bla KPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla NDM and bla OXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132 K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying bla KPC, bla NDM, and/or bla OXA-48. On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring bla IMP and bla VIM. We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories.
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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Proteins / Beta-Lactamases / Klebsiella Infections / Immunoassay / Klebsiella pneumoniae Type of study: Diagnostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Turkey Institution/Affiliation country: Acibadem University ,/TR / Marmara University/TR

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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Proteins / Beta-Lactamases / Klebsiella Infections / Immunoassay / Klebsiella pneumoniae Type of study: Diagnostic study Limits: Humans Country/Region as subject: Asia Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Turkey Institution/Affiliation country: Acibadem University ,/TR / Marmara University/TR