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Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
Zepeda, Andrea B; Pessoa Jr, Adalberto; Farías, Jorge G.
  • Zepeda, Andrea B; Universidad de La Frontera. Facultad de Ingeniería, Ciencias y Administración. Departamento de Ingeniería Química. Temuco. CL
  • Pessoa Jr, Adalberto; Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Tecnologia Bioquímico-Farmacêutica. São Paulo. BR
  • Farías, Jorge G; Universidad de La Frontera. Facultad de Ingeniería, Ciencias y Administración. Departamento de Ingeniería Química. Temuco. CL
Braz. j. microbiol ; 49(supl.1): 119-127, 2018. tab, graf
Article in English | LILACS | ID: biblio-974317
ABSTRACT
Abstract Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others; but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Pichia / Recombinant Proteins / Carbon / Promoter Regions, Genetic Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Brazil / Chile Institution/Affiliation country: Universidad de La Frontera/CL / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Pichia / Recombinant Proteins / Carbon / Promoter Regions, Genetic Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Brazil / Chile Institution/Affiliation country: Universidad de La Frontera/CL / Universidade de São Paulo/BR