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Evaluation of chemokines and receptors in gnotobiotic root canal infection by F. nucleatum and E. faecalis
Department of Restorative DentistrySanta-Rosa, Caroline Christine; Department of Restorative DentistryThebit, Marcela Marçal; Department of Restorative DentistryMaciel, Kamilla Faria; Brito, Luciana Carla Neves de; Department of Biochemistry and ImmunologyVieira, Leda Quercia; Department of Restorative DentistryRibeiro-Sobrinho, Antônio Paulino.
  • Department of Restorative DentistrySanta-Rosa, Caroline Christine; Universidade Federal de Minas Gerais - UFMG. Faculty of Dentistry. Department of Restorative DentistrySanta-Rosa, Caroline Christine. Belo Horizonte. BR
  • Department of Restorative DentistryThebit, Marcela Marçal; Universidade Federal de Minas Gerais - UFMG. Faculty of Dentistry. Department of Restorative DentistryThebit, Marcela Marçal. Belo Horizonte. BR
  • Department of Restorative DentistryMaciel, Kamilla Faria; Universidade Federal de Minas Gerais - UFMG. Faculty of Dentistry. Department of Restorative DentistryMaciel, Kamilla Faria. Belo Horizonte. BR
  • Brito, Luciana Carla Neves de; Fundação Universidade de Itaúna - FUI. Faculty of Dentistry. Itaúna. BR
  • Department of Biochemistry and ImmunologyVieira, Leda Quercia; Universidade Federal de Minas Gerais - UFMG. Institute of Biological Sciences. Department of Biochemistry and ImmunologyVieira, Leda Quercia. Belo Horizonte. BR
  • Department of Restorative DentistryRibeiro-Sobrinho, Antônio Paulino; Universidade Federal de Minas Gerais - UFMG. Faculty of Dentistry. Department of Restorative DentistryRibeiro-Sobrinho, Antônio Paulino. Belo Horizonte. BR
Braz. oral res. (Online) ; 32: e120, 2018. tab, graf
Article in English | LILACS | ID: biblio-974436
ABSTRACT
Abstract The present study aims to evaluate the longitudinal effects of induced experimental infections in gnotoxenic animals on the expression of inflammatory chemokines and their receptors in periradicular tissues. The null hypothesis tested was that Enterococcus faecalis and Fusobacterium nucleatum had no effect on CCR5, CCL5, CXCL10, CCL2/MCP-1, CXCR2 and CCR1 expression. Two groups of five animals (n = 5) aged between 8 and 12 weeks were used in this study. The animals were anaesthetized, and coronary access was performed in the first molar on the right and left sides. Microorganisms were inoculated into the left molar, and the right molar was sealed without contamination to function as a control. Animals were sacrificed 7 and 14 days after infection, and periapical tissues were collected. The cytokine mRNA expression levels were assessed using real-time PCR. The chemokine mRNA expression levels demonstrated that the experimental infection was capable of inducing increased chemokine expression on day 7 compared to that on day 14, except for CCR5 and CCL5, which showed no changes. The gnotoxenic animal model proved to be effective and allowed evaluation of the immune response against a known infection. Additionally, this study demonstrates that gene expression of chemokines and their receptors against the experimental infection preferentially prevailed during the initial phase of induction of the periradicular alteration (i.e., on day 7 post-infection).
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Full text: Available Index: LILACS (Americas) Main subject: Gram-Positive Bacterial Infections / Chemokines / Receptors, Chemokine / Dental Pulp Cavity / Dental Pulp Diseases / Fusobacterium Infections / Germ-Free Life Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Universidade de Itaúna - FUI/BR / Universidade Federal de Minas Gerais - UFMG/BR

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Full text: Available Index: LILACS (Americas) Main subject: Gram-Positive Bacterial Infections / Chemokines / Receptors, Chemokine / Dental Pulp Cavity / Dental Pulp Diseases / Fusobacterium Infections / Germ-Free Life Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Universidade de Itaúna - FUI/BR / Universidade Federal de Minas Gerais - UFMG/BR