Experimental furcal perforation treated with mineral trioxide aggregate plus selenium: immune response
Braz. oral res. (Online)
;
32: e103, 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-974462
ABSTRACT
Abstract The aim of this study is to evaluate the expression of cytokines in response to mineral trioxide aggregate (MTA) plus selenium in germ-free mice with experimental furcal perforation. The first left maxillary molar was opened, and the furcal area was perforated and treated with post-MTA-Se (experimental group). The same surgical intervention was performed for the maxillary right first molar, which was treated with MTA (control group). Fifteen mice were sacrificed 7, 14, and 21 days after furcal perforation, and periapical tissue samples were collected. The mRNA expression levels of the cytokines TGF-β, TNF-α, IFN-γ, HPRT, IL-10, IL-4, RANK, RANKL, IL-1, and IL-17 were assessed by using real-time polymerase chain reaction. In the experimental group, at 21-days post-MTA-Se sealing, the mRNA levels of TNF-α and IL-10 were upregulated compared with those in the control group (p < 0.05). Futher assessment revealed basal mRNA expression levels of IL-1α, IFN-γ, RANK, RANKL, IL-17A, IL-4, and TGF-β, over long experimental times, in both the experimental and control groups (p > 0.05). In conclusion, MTA+Se sealing favoured increased expression of IL-10 and TNF-α at later time points (day 21).
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Oxides
/
Root Canal Filling Materials
/
Selenium
/
Cytokines
/
Silicates
/
Furcation Defects
/
Calcium Compounds
/
Aluminum Compounds
/
Dental Pulp Cavity
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Braz. oral res. (Online)
Journal subject:
Dentistry
Year:
2018
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
The Forsyth Institute/US
/
Universidade Federal de Minas Gerais/BR
/
Universidade de Itaúna/BR
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