Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence
Clinics
;
73: e465, 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-974922
ABSTRACT
OBJECTIVE:
The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil.METHOD:
One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region.RESULTS:
Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples.CONCLUSIONS:
The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Breast Neoplasms
/
DNA, Viral
/
Papillomavirus Infections
Type of study:
Observational study
/
Prevalence study
/
Risk factors
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2018
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Evandro Chagas/BR
/
Universidade Federal Ceara (UFC)/BR
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