Beneficial effects of Oltipraz, nuclear factor - erythroid - 2 - related factor 2 (Nrf2), on renal damage in unilateral ureteral obstruction rat model
Int. braz. j. urol
;
44(6): 1243-1251, Nov.-Dec. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-975668
ABSTRACT
ABSTRACT Introduction:
We investigated whether Oltipraz (OPZ) attenuated renal fibrosis in a unilateral ureteral obstruction (UUO) rat model. Materials andMethods:
We randomly divided 32 rats into four groups, each consisting of eight animals as follows Rats in group 1 underwent a sham operation and received no treatment. Rats in group 2 underwent a sham operation and received OPZ. Rats in group 3 underwent unilateral ureteral ligation and received no treatment. Group 4 rats were subjected to unilateral ureteral ligation plus OPZ administration. Transforming growth factor beta-1 (TGF-β1), E-cadherin, nitric oxide (NO) and hydroxyproline levels were measured. Histopathological and immunohistochemical examinations were carried out.Results:
TGF-β1, NO and E-cadherin levels in the UUO group were significantly higher than the sham group and these values were significantly different in treated groups compared to the UUO group. In rats treated with UUO + OPZ, despite the presence of mild tubular degeneration and less severe tubular necrosis, glomeruli maintained a better morphology when compared to the UUO group. Expressions of α-SMA in immunohistochemistry showed that the staining positivity decreased in the tubules of the OPZ-treated group.Conclusions:
While the precise mechanism of action remains unknown, our results demonstrated that OPZ exerted a protective role in the UUO-mediated renal fibrosis rat model highlighting a promising therapeutic potency of Nrf2-activators for alleviating the detrimental effects of unilateral obstruction in kidneys.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Pyrazines
/
Ureteral Obstruction
/
NF-E2-Related Factor 2
/
Kidney Diseases
Type of study:
Etiology study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2018
Type:
Article
Affiliation country:
Turkey
Institution/Affiliation country:
Catalca Ilyas Cokay State Hospital/TR
/
Istanbul Medeniyet University/TR
/
Istanbul University/TR
/
Kocaeli University/TR
/
University of Health Sciences/TR
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