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Adhesion and Initial Colonization of Streptococcus mutans is Influenced by Time and Composition of Different Composites / La Adhesión y la Colonización Inicial de Streptococcus mutans están Asociadas al Tiempo y la Composición de Diferentes Compuestos
Department of Pediatric DentistrySilva, Raquel A. B; Department of Pediatric DentistryNelson-Filho, Paulo; Department of Pediatric DentistryDe-Oliveira, Katharina M. H; Department of Pediatric DentistryRomualdo, Priscilla C; Gatón-Hernandez, Patrícia; Aires, Carolina P; Department of Pediatric DentistrySilva, Lea A. B.
  • Department of Pediatric DentistrySilva, Raquel A. B; University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric DentistrySilva, Raquel A. B. Ribeirão Preto. BR
  • Department of Pediatric DentistryNelson-Filho, Paulo; University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric DentistryNelson-Filho, Paulo. Ribeirão Preto. BR
  • Department of Pediatric DentistryDe-Oliveira, Katharina M. H; University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric DentistryDe-Oliveira, Katharina M. H. Ribeirão Preto. BR
  • Department of Pediatric DentistryRomualdo, Priscilla C; University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric DentistryRomualdo, Priscilla C. Ribeirão Preto. BR
  • Gatón-Hernandez, Patrícia; University of Barcelona. School of Dentistry. Department of Integrated Pediatric Dentistry. Barcelona. ES
  • Aires, Carolina P; University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Physics and Chemistry. São Paulo. BR
  • Department of Pediatric DentistrySilva, Lea A. B; University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric DentistrySilva, Lea A. B. Ribeirão Preto. BR
Int. j. odontostomatol. (Print) ; 12(4): 395-400, dic. 2018. tab, graf
Article in English | LILACS | ID: biblio-975763
ABSTRACT
ABSTRACT The aim of this study was to evaluate the adhesion and initial colonization of S. mutans on the surface of novel composite resins during three experimental periods. Biofilms were formed on specimens of Enamel Plus HRI (Hri), FiltekTM Silorane (Si), Tetric EvoCeram® Bulk Fill (Te), KaloreTM (K), and FiltekTM Z250 (Z) (n=4/experimental period). After 4, 8 and 24 hours, the specimens were assessed for bacterial colony forming unit (CFU/mm2) levels and scanned by electron microscopy. All material surfaces showed a similar susceptibility to bacterial adhesion at 4 hours (p>0.05). The amount of microorganisms in the formed biofilms increased at 8 hours for all groups (p<0.05) and decreased at 24 hours only for Te and Z groups (p<0.05). After 24 h, the K group showed higher microorganism counts compared to the other groups. All composite resins evaluated were susceptible to adhesion by streptococci. However, bacteria attachment was not sufficient to maintain S. mutans colonization on Tetric EvoCeram® Bulk Fill and FiltekTM Z250 composites. Composite K presented the highest mean values for bacterial adhesion. Also, it was observed that the composition of resins could interfere with colonization mechanisms.
RESUMEN
RESUMEN El objetivo de este estudio fue evaluar la adhesión y la colonización inicial de S. mutans en la superficie de nuevas resinas compuestas durante tres períodos experimentales. Las biopelículas se formaron en muestras de Enamel Plus HRI (Hri), FiltekTM Silorane (Si), Tetric EvoCeram® Bulk Fill (Te), KaloreTM (K) y FiltekTM Z250 (Z) (n = 4 / período experimental). Después de 4, 8 y 24 horas, las muestras se evaluaron para determinar los niveles de la unidad formadora de colonias bacterianas (UFC / mm2) y se escanearon por microscopía electrónica. Todas las superficies del material mostraron una susceptibilidad similar a la adhesión bacteriana a las 4 horas (p> 0,05). La cantidad de microorganismos en las biopelículas formadas aumentó a las 8 horas para todos los grupos (p <0,05) y disminuyó a las 24 horas solo para los grupos Te y Z (p <0,05). Después de 24 horas, el grupo K mostró mayores recuentos de microorganismos en comparación con los otros grupos. Todas las resinas compuestas evaluadas fueron susceptibles a la adhesión por estreptococos. Sin embargo, la unión de bacterias no fue suficiente para mantener la colonización de S. mutans en los compuestos Tetric EvoCeram® Bulk Fill y FiltekTM Z250. El compuesto K presentó los valores medios más altos para la adhesión bacteriana. Además, se observó que la composición de las resinas podría interferir con los mecanismos de colonización.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Dental Caries / Dental Plaque Limits: Humans Language: English Journal: Int. j. odontostomatol. (Print) Journal subject: Dentistry Year: 2018 Type: Article Affiliation country: Brazil / Spain Institution/Affiliation country: University of Barcelona/ES / University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Dental Caries / Dental Plaque Limits: Humans Language: English Journal: Int. j. odontostomatol. (Print) Journal subject: Dentistry Year: 2018 Type: Article Affiliation country: Brazil / Spain Institution/Affiliation country: University of Barcelona/ES / University of São Paulo/BR