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Proteomic analysis of the acquired enamel pellicle formed on human and bovine tooth: a study using the Bauru in situ pellicle model (BISPM)
Pelá, Vinícius Taioqui; Cassiano, Luiza Paula Silva; Departmento de Ciências BiológicasVentura, Talita Mendes da Silva; Departmento de Ciências BiológicasSouza-e-Silva, Cintia Maria de; Departmento de Ciências BiológicasGironda, Carlos Condarco; Departmento de Ciências BiológicasRios, Daniela; Departmento de Ciências BiológicasBuzalaf, Marília Afonso Rabelo.
  • Pelá, Vinícius Taioqui; Universidade Federal de São Carlos. Departmento de Genetica e Evolução. São Carlos. BR
  • Cassiano, Luiza Paula Silva; Faculdades Integradas Aparício Carvalho. Porto Velho. BR
  • Departmento de Ciências BiológicasVentura, Talita Mendes da Silva; Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departmento de Ciências BiológicasVentura, Talita Mendes da Silva. Bauru. BR
  • Departmento de Ciências BiológicasSouza-e-Silva, Cintia Maria de; Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departmento de Ciências BiológicasSouza-e-Silva, Cintia Maria de. Bauru. BR
  • Departmento de Ciências BiológicasGironda, Carlos Condarco; Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departmento de Ciências BiológicasGironda, Carlos Condarco. Bauru. BR
  • Departmento de Ciências BiológicasRios, Daniela; Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departmento de Ciências BiológicasRios, Daniela. Bauru. BR
  • Departmento de Ciências BiológicasBuzalaf, Marília Afonso Rabelo; Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departmento de Ciências BiológicasBuzalaf, Marília Afonso Rabelo. Bauru. BR
J. appl. oral sci ; 27: e20180113, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-975875
ABSTRACT
Abstract The acquired enamel pellicle (AEP) is an organic film, bacteria-free, formed in vivo as a result of the selective adsorption of salivary proteins and glycoproteins to the solid surfaces exposed to the oral environment.

Objective:

This study aimed to compare the proteomic profile of AEP formed in situ on human and bovine enamel using a new intraoral device (Bauru in situ pellicle model - BISPM). Material and

Methods:

One hundred and eight samples of human and bovine enamel were prepared (4×4 mm). Nine subjects with good oral conditions wore a removable jaw appliance (BISPM) with 6 slabs of each substrate randomly allocated. The AEP was formed during the morning, for 120 minutes, and collected with an electrode filter paper soaked in 3% citric acid. This procedure was conducted in triplicate and the pellicle collected was processed for analysis by LC-ESI-MS/MS. The obtained mass spectrometry MS/MS spectra were searched against human protein database (SWISS-PROT).

Results:

The use of BISPM allowed the collection of enough proteins amount for proper analysis. A total of 51 proteins were found in the AEP collected from the substrates. Among them, 15 were common to both groups, 14 were exclusive of the bovine enamel, and 22 were exclusive of the human enamel. Proteins typically found in the AEP were identified, such as Histatin-1, Ig alpha-1, Ig alpha 2, Lysozyme C, Statherin and Submaxillary gland androgen-regulated protein 3B. Proteins not previously described in the AEP, such as metabolism, cell signaling, cell adhesion, cell division, transport, protein synthesis and degradation were also identified.

Conclusion:

These results demonstrate that the proteins typically found in the AEP appeared in both groups, regardless the substrate. The BISPM revealed to be a good device to be used in studies involving proteomic analysis of the AEP.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Proteins / Dental Pellicle Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdades Integradas Aparício Carvalho/BR / Universidade Federal de São Carlos/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Proteins / Dental Pellicle Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdades Integradas Aparício Carvalho/BR / Universidade Federal de São Carlos/BR / Universidade de São Paulo/BR