First isolation of West Nile virus in Brazil
Mem. Inst. Oswaldo Cruz
;
114: e180332, 2019. graf
Article
in English
| LILACS
| ID: biblio-976238
ABSTRACT
BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000's decade.
Full text:
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Index:
LILACS (Americas)
Main subject:
Brazil
/
Horses
Type of study:
Prognostic study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2019
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Evandro Chagas/BR
/
Ministério da Saúde/BR
/
Secretaria de Estado da Saúde do Espírito Santo/BR
/
Secretaria de Saúde do Município de Venécia/BR
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