Your browser doesn't support javascript.
loading
A 14-20 kDa protein binds to the upstream region of the phtM operon involved in the synthesis of phaseolotoxin in Pseudomonas syringae pv. phaseolicola NPS3121 / Una proteína de 14-20 kDa se une a la región río arriba del operón phtM involucrado en la síntesis de faseolotoxina en Pseudomonas syringae pv. phaseolicola NPS3121
Alvarez-Morales, Ariel; Henández-Morales, Alejandro; Secretaría de Investigación y PosgradoArvizu-Gomez, Jackeline L.
  • Alvarez-Morales, Ariel; IPN. Centro de Investigación y de Estudios Avanzados. Irapuato. MX
  • Henández-Morales, Alejandro; Universidad Autónoma de San Luis Potosí. Unidad Académica Multidisciplinaria Zona Huasteca. San Luis Potosí. MX
  • Secretaría de Investigación y PosgradoArvizu-Gomez, Jackeline L; Universidad Autónoma de Nayarit. Centro de Innovación y Transferencia de Tecnología. Secretaría de Investigación y PosgradoArvizu-Gomez, Jackeline L. Tepic. MX
Rev. argent. microbiol ; 50(2): 115-125, jun. 2018. ilus, tab
Article in English | LILACS | ID: biblio-977229
ABSTRACT
Pseudomonas syringae pv. phaseolicola is a phytopathogenic bacterium in beans that produces a phytotoxin called phaseolotoxin, in whose synthesis a group of genes that belong to the "Pht cluster" are involved. This cluster comprises 23 genes arranged in 5 transcriptional units, two monocistronic (argK, phtL) and three polycistronic (phtA, phtD, phtM) operons, whose expression is increased at 18°C, correlating with the production of phaseolotoxin by the bacterium. So far, the regulatory mechanisms involved in phaseolotoxin synthesis are poorly understood and only the requirement of low temperatures for its synthesis has been demon strated. Therefore, in this study we searched for regulatory proteins that could be involved in the phaseolotoxin synthesis, focusing on the regulation of the phtM operon. Gel shift assays showed that the promoter region of the phtM operon contains binding sites for putative regulatory proteins, which are encoded outside the Pht cluster and are independent of the GacS-GacA two-component system. Deletion assays with the promoter region of the phtM operon show that the binding site for a putative transcription factor is located within a 58 bp region. The putative transcription factor of the phtM operon has an apparent molecular mass in the 14-20 kDa range. Furthermore, the results demonstrate that the transcription factor recognizes and binds the upstream phtM region as monomer o multimer of a single polypeptide. Our findings provide new insights into the regulatory mechanisms involved in phaseolotoxin production, and suggest that the Pht cluster was integrated into the global regulatory mechanism of P. syringae pv. phaseolicola.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Operon / Ornithine / Pseudomonas syringae Language: English Journal: Rev. argent. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Mexico Institution/Affiliation country: IPN/MX / Universidad Autónoma de Nayarit/MX / Universidad Autónoma de San Luis Potosí/MX

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Operon / Ornithine / Pseudomonas syringae Language: English Journal: Rev. argent. microbiol Journal subject: Microbiology Year: 2018 Type: Article Affiliation country: Mexico Institution/Affiliation country: IPN/MX / Universidad Autónoma de Nayarit/MX / Universidad Autónoma de San Luis Potosí/MX