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Serological study of brucellosis in Argentine Creole sheep / Estudio serológico de brucelosis en ovejas criollas de Argentina
López, Gustavo E; Peña, Sabrinan; Escobar, Gabriela I; Hasan, Déborah B; Lucero, Nidia E.
Affiliation
  • López, Gustavo E; Lomas de Zamora National University. Agricultural Sciences Faculty. Llavallol. AR
  • Peña, Sabrinan; Lomas de Zamora National University. Agricultural Sciences Faculty. Llavallol. AR
  • Escobar, Gabriela I; National Laboratories and Institutes of Health Administration (ANLIS) Dr. C. G. Malbran. Brucellosis Service. Buenos Aires. AR
  • Hasan, Déborah B; National Laboratories and Institutes of Health Administration (ANLIS) Dr. C. G. Malbran. Brucellosis Service. Buenos Aires. AR
  • Lucero, Nidia E; National Laboratories and Institutes of Health Administration (ANLIS) Dr. C. G. Malbran. Brucellosis Service. Buenos Aires. AR
Rev. argent. microbiol ; Rev. argent. microbiol;50(3): 285-289, set. 2018. map, tab
Article in En | LILACS | ID: biblio-977245
Responsible library: BR1.1
ABSTRACT
Ovine cattle was introduced into America during the Spanish conquest with the second journey of Columbus to the Antilles and was disseminated throughout the region. In 1587, sheep were introduced into Argentina, later developing into the "Creole" breed. We selected 486 animals from different Argentine provinces with the aim of determining the serological status of brucellosis caused by Brucella melitensis and Brucella ovis. For the detection of antibodies against smooth Brucella spp., the Rose Bengal test (RBT) was performed as screening test while the serum agglutination test (SAT) and 2 mercapto-ethanol (2ME) were run as a confirmatory technique. Moreover, for the detection of antibodies against rough Brucella spp., we used the rapid slide agglutination test (RSAT) for screening and an indirect ELISA (IELISA) as confirmatory assay. This study showed that the total positive percentage of brucellosis due to B. ovis was 2.9%. Excluding the animals mixed with the Suffolk breed; seropositivity would be 0.6%. All animals tested negative for brucellosis caused by B. melitensis.
RESUMEN
El ganado ovino fue introducido en América durante el segundo viaje de Colón a las Antillas y rápidamente se diseminó por la región. En 1587 entraron a la Argentina las primeras ovejas, estas se adaptaron a las condiciones naturales y se desarrolló así la denominada «oveja criolla¼. Se seleccionaron 486 animales de diferentes provincias para conocer el estado serológico de la enfermedad producida por Brucella melitensis y Brucella ovis. Para detectar anticuerpos anti-Brucella en fase lisa se utilizó la prueba de rosa de Bengala (RBT) como tamiz y como confirmatorias seroaglutinación lenta en tubo (SAT) y 2-mercaptoetanol (2ME). En fase rugosa se realizó como tamiz la prueba rápida de aglutinación (RSAT) y ELISA indirecto como prueba confirmatoria (IELISA). Este estudio mostró un porcentaje de seropositividad de brucelosis causada por B. ovis del 2,9%. Excluyendo los animales cruzados con la raza Suffolk, la seropositividad sería del 0,6%. Todos los animales fueron negativos a brucelosis causada por B. melitensis.
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Full text: 1 Index: LILACS Main subject: Sheep Diseases / Brucellosis / Antibodies, Bacterial Type of study: Diagnostic_studies Limits: Animals Country/Region as subject: America do sul / Argentina Language: En Journal: Rev. argent. microbiol Journal subject: MICROBIOLOGIA Year: 2018 Type: Article

Full text: 1 Index: LILACS Main subject: Sheep Diseases / Brucellosis / Antibodies, Bacterial Type of study: Diagnostic_studies Limits: Animals Country/Region as subject: America do sul / Argentina Language: En Journal: Rev. argent. microbiol Journal subject: MICROBIOLOGIA Year: 2018 Type: Article