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The apoptotic, angiogenic and cell proliferation genes CD63, S100A6 e GNB2L1 are altered in patients with endometriosis / Os genes apoptóticos, angiogênicos e de proliferação celular CD63, S100A6 e GNB2L1 estão alterados em pacientes com endometriose
Department of Gynecology and ObstetricsGomes, Valéria Aguiar; Department of Gynecology and ObstetricsBonocher, Camila de Moraes; Department of Gynecology and ObstetricsRosa-e-Silva, Júlio César; Department of GeneticsPaz, Cláudia Cristina Paro de; Department of Gynecology and ObstetricsFerriani, Rui Alberto; Department of Gynecology and ObstetricsMeola, Juliana.
  • Department of Gynecology and ObstetricsGomes, Valéria Aguiar; Universidade de São Paulo. School of Medicine of Ribeirao Preto. Department of Gynecology and ObstetricsGomes, Valéria Aguiar. Ribeirão Preto. BR
  • Department of Gynecology and ObstetricsBonocher, Camila de Moraes; Universidade de São Paulo. School of Medicine of Ribeirao Preto. Department of Gynecology and ObstetricsBonocher, Camila de Moraes. Ribeirão Preto. BR
  • Department of Gynecology and ObstetricsRosa-e-Silva, Júlio César; Universidade de São Paulo. School of Medicine of Ribeirao Preto. Department of Gynecology and ObstetricsRosa-e-Silva, Júlio César. Ribeirão Preto. BR
  • Department of GeneticsPaz, Cláudia Cristina Paro de; Universidade de São Paulo. School of Medicine of Ribeirao Preto. Department of GeneticsPaz, Cláudia Cristina Paro de. Ribeirão Preto. BR
  • Department of Gynecology and ObstetricsFerriani, Rui Alberto; Universidade de São Paulo. School of Medicine of Ribeirao Preto. Department of Gynecology and ObstetricsFerriani, Rui Alberto. Ribeirão Preto. BR
  • Department of Gynecology and ObstetricsMeola, Juliana; Universidade de São Paulo. School of Medicine of Ribeirao Preto. Department of Gynecology and ObstetricsMeola, Juliana. Ribeirão Preto. BR
Rev. bras. ginecol. obstet ; 40(10): 606-613, Oct. 2018. graf
Article in English | LILACS | ID: biblio-977778
ABSTRACT
Abstract Objective The aim of the present study was to analyze the expression of the CD63, S100A6, and GNB2L1genes, which participate in mechanisms related to the complex pathophysiology of endometriosis. Methods A case-control study was conducted with 40 women who were diagnosed with endometriosis, and 15 fertile and healthy women. Paired samples of eutopic endometrium and endometriotic lesions (peritoneal and ovarian endometriotic implants) were obtained from the women with endometriosis in the proliferative (n = 20) or secretory phases (n = 20) of the menstrual cycle. As controls, paired endometrial biopsy samples were collected from the healthy women in the proliferative (n = 15) and secretory (n = 15) phases of the samemenstrual cycle.We analyzed the expression levels of the CD63, S100A6, and GNB2L1 genes by real-time polymerase chain reaction. Results An increase in CD63, S100A6, and GNB2L1 gene transcript levels was observed in the ectopic implants compared with the eutopic endometrium of the women with and without endometriosis, regardless of the phase of the menstrual cycle. Conclusion These findings suggest that the CD63, S100A6, and GNB2L1 genesmay be involved in the pathogenesis of endometriosis, since they participate in mechanisms such as inhibition of apoptosis, angiogenesis and cell proliferation, which lead to the loss of cell homeostasis in the ectopic endometrium, thus contributing to the implantation and survival of the tissue in the extrauterine environment.
RESUMO
Resumo Objetivo O objetivo do presente estudo foi analisar a expressão dos genes CD63, S100A6 e GNB2L1, que participam em mecanismos relacionados à complexa fisiopatologia da endometriose. Métodos Um estudo caso-controle foi realizado com 40 mulheres diagnosticadas com endometriose e 15 mulheres férteis e saudáveis. Amostras pareadas de endométrio eutópico e de lesões endometrióticas (implantes endometrióticos peritoneais e ovarianos) foram obtidas de mulheres com endometriose nas fases proliferativa (n = 20) ou secretora (n = 20) do ciclo menstrual. Como controle, amostras pareadas de biópsia endometrial foram coletadas de mulheres saudáveis nas fases proliferativa (n = 15) e secretora (n = 15) nomesmo ciclomenstrual. Foram analisados os níveis de expressão dos genes CD63, S100A6 e GNB2L1 por reação em cadeia da polimerase em tempo real. Resultados Foi observado um aumento nos níveis de transcritos dos genes CD63, S100A6 e GNB2L1 em implantes ectópicos quando comparado ao endométrio eutópico de mulheres com e sem endometriose, independente da fase do ciclo menstrual. Conclusão Estes achados sugerem que os genes CD63, S100A6 e GNB2L1 podem estar envolvidos na patogênese da endometriose, pois participam de mecanismos como inibição de apoptose, angiogênese e proliferação celular, os quais levam à perda da homeostase celular no endométrio ectópico e, portanto, contribuem para o implante e a sobrevivência do tecido no ambiente extrauterino.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Apoptosis / Cell Cycle Proteins / Cell Proliferation / Endometriosis / Tetraspanin 30 / S100 Calcium Binding Protein A6 / Receptors for Activated C Kinase / Neoplasm Proteins / Neovascularization, Pathologic Type of study: Observational study Limits: Adult / Female / Humans Language: English Journal: Rev. bras. ginecol. obstet Journal subject: Gynecology / Obstetrics Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Apoptosis / Cell Cycle Proteins / Cell Proliferation / Endometriosis / Tetraspanin 30 / S100 Calcium Binding Protein A6 / Receptors for Activated C Kinase / Neoplasm Proteins / Neovascularization, Pathologic Type of study: Observational study Limits: Adult / Female / Humans Language: English Journal: Rev. bras. ginecol. obstet Journal subject: Gynecology / Obstetrics Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR