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Bone callus formation is highly disrupted by dietary restriction in growing rats sustaining a femoral fracture
Botega, Iara Inácio; Zamarioli, Ariane; Guedes, Patrícia Madalena San Gregório; Silva, Raquel Assed Bezerra da; Issa, João Paulo Mardegan; Butezloff, Mariana Maloste; Sousa, Yara Terezinha Corrêa Silva; Ximenez, João Paulo Bianchi; Volpon, José Batista.
  • Botega, Iara Inácio; Universidade de São Paulo. School of Medicine. Postgraduate Program in Health Sciences Applied to the Locomotor System. Ribeirao Preto. BR
  • Zamarioli, Ariane; USP. School of Medicine. Laboratory of Bioengineering. Ribeirao Preto. BR
  • Guedes, Patrícia Madalena San Gregório; USP. School of Medicine. Postgraduate Program in Health Sciences Applied to the Locomotor System. Ribeirao Preto. BR
  • Silva, Raquel Assed Bezerra da; USP. School of Dentistry. Department of Children's Clinic. Ribeirao Preto. BR
  • Issa, João Paulo Mardegan; USP. School of Dentistry. Department of Morphology, Physiology and Basic Pathology. Ribeirao Preto. BR
  • Butezloff, Mariana Maloste; USP. School of Medicine. Postgraduate Program in Health Sciences Applied to the Locomotor System. Ribeirao Preto. BR
  • Sousa, Yara Terezinha Corrêa Silva; Universidade de Ribeirão Preto. School of Dentistry. BR
  • Ximenez, João Paulo Bianchi; USP. School of Pharmaceutical Sciences. Postgraduate Program in Toxicology. Ribeirao Preto. BR
  • Volpon, José Batista; USP. School of Medicine. Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System. Ribeirao Preto. BR
Acta cir. bras ; 34(1): e20190010000002, 2019. graf
Article in English | LILACS | ID: biblio-983685
ABSTRACT
Abstract

Purpose:

To evaluate the effects of food restriction on fracture healing in growing rats.

Methods:

Sixty-eight male Wistar rats were assigned to two groups (1) Control and (2) Dietary restriction. After weaning the dietary restricted animals were fed ad libitum for 42 days with 50% of the standard chow ingested by the control group. Subsequently, the animals underwent bone fracture at the diaphysis of the right femur, followed by surgical stabilization of bone fragments. On days 14 and 28 post-fracture, the rats were euthanized, and the fractured femurs were dissected, the callus was analyzed by dual-energy X-ray absorptiometry, micro-computed tomography, histomorphometry, mechanical tests, and gene expression.

Results:

Dietary restriction decreased body mass gain and resulted in several phenotypic changes at the bone callus (a delay in cell proliferation and differentiation, lower rate of newly formed bone and collagen deposition, reductions in bone callus density and size, decrease in tridimensional callus volume, deterioration in microstructure, and reduction in bone callus strength), together with the downregulated expression of osteoblast-related genes.

Conclusion:

Dietary restriction had detrimental effects on osseous healing, with a healing delay and a lower quality of bone callus formation.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Bony Callus / Bone Density / Fracture Healing / Malnutrition / Femoral Fractures / Fractures, Closed Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: USP/BR / Universidade de Ribeirão Preto/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bony Callus / Bone Density / Fracture Healing / Malnutrition / Femoral Fractures / Fractures, Closed Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: USP/BR / Universidade de Ribeirão Preto/BR / Universidade de São Paulo/BR