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Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides / Impacto das bactérias solubilizantes de fosfato no trigo (Triticum aestivum ) na presença de pesticidas
Munir, I; Bano, A; Faisal, M.
  • Munir, I; University of the Punjab. Department of Microbiology and Molecular Genetics. Lahore. PK
  • Bano, A; University of the Punjab. Department of Microbiology and Molecular Genetics. Lahore. PK
  • Faisal, M; University of the Punjab. Department of Microbiology and Molecular Genetics. Lahore. PK
Braz. j. biol ; 79(1): 29-37, Jan.-Mar 2019. tab, graf
Article in English | LILACS | ID: biblio-984009
ABSTRACT
Abstract Three phosphate solubilizing bacteria were isolated and identified by 16S rRNA sequencing as Pseudomonas putida, Pseudomonas sp and Pseudomonas fulva . The strains were subjected to plant biochemical testing and all the PGPR attributes were checked in the presence of pesticides (chlorpyrifos and pyriproxyfen). The phosphate solubilizing index of strain Ros2 was highest in NBRIP medium i.e 2.23 mm. All the strains showed acidic pH (ranges from 2.5-5) on both medium i.e PVK and NBRIP. Strain Ros2 was highly positive for ammonia production as well as siderophore production while strain Rad2 was positive for HCN production. The results obtained by the strains Rad1, Rad2 and Ros2 for auxin production were 33.1, 30.67 and 15.38 µg ml-1, respectively. Strain Rad1 showed 16% increase in percentage germination in comparison to control in the presence of pesticide stress. Most promising results for chlorophyll content estimation were obtained in the presence of carotenoids upto 6 mgg-1 without stress by both strains Rad1 and Rad2. Study suggests that especially strain Ros2 can enhance plant growth parameters in the pesticide stress.
RESUMO
Resumo Três bactérias solubilizantes de fosfato foram isoladas e identificadas por seqüenciamento de rRNA 16S como Pseudomonas putida, Pseudomonas sp e Pseudomonas fulva. As estirpes foram submetidas a testes bioquímicos de plantas e todos os atributos PGPR foram verificados na presença de pesticidas (clorpirifos e piriproxifeno). O índice de solubilização de fosfato da estirpe Ros2 foi mais elevado no meio NBRIP, isto é, 2,23 mm. Todas as estirpes apresentaram um pH ácido (varia de 2,5-5) em ambos os meios, isto é PVK e NBRIP. A estirpe Ros2 foi altamente positiva para a produção de amoníaco, bem como a produção de sideróforos enquanto a estirpe Rad2 foi positiva para a produção de HCN. Os resultados obtidos pelas estirpes Rad1, Rad2 e Ros2 para a produção de auxina foram 33,1, 30,67 e 15,38 μg ml-1 , respectivamente. A deformação Rad1 mostrou aumento de 16% na germinação percentual em comparação com o controlo na presença de stress de pesticida. Os resultados mais promissores para a estimativa do teor de clorofila foram obtidos na presença de carotenóides até 6 mgg-1 sem estresse por ambas as cepas Rad1 e Rad2. Estudo sugere que especialmente a estirpe Ros2 pode melhorar parâmetros de crescimento de plantas no estresse de pesticidas.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Phosphates / Pseudomonas / Pyridines / Triticum / Chlorpyrifos / Insecticides Country/Region as subject: Asia Language: English Journal: Braz. j. biol Journal subject: Biology Year: 2019 Type: Article Affiliation country: Pakistan Institution/Affiliation country: University of the Punjab/PK

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Full text: Available Index: LILACS (Americas) Main subject: Phosphates / Pseudomonas / Pyridines / Triticum / Chlorpyrifos / Insecticides Country/Region as subject: Asia Language: English Journal: Braz. j. biol Journal subject: Biology Year: 2019 Type: Article Affiliation country: Pakistan Institution/Affiliation country: University of the Punjab/PK