Hyaluronic acid in tobacco-exposed rats. Inflammatory reaction, and duration of effect
Acta cir. bras
;
34(2): e201900202, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-989055
ABSTRACT
Abstract Purpose:
To evaluate the hyaluronic acid (HA) inflammatory reaction, fibroblasts, fibrosis and duration of effect in the dorsal region of tobacco-exposed rats.Methods:
Ten Wistar rats were divided into two groups tobacco-exposed-group (TEG;n=5) and air-control-group (CG;n=5). The TEG animals were tobacco-exposed twice a day, 30-minutes/session, during 60 days. After this period, all animals received 0.1 mL HA subcutaneous injection in the dorsal area. The volume of HA was measured immediately after HA injection and weekly using a hand-caliper in nine weeks. After this period, all the animals were euthanized, and a specimen of was collected to evaluate inflammatory cells, fibroblasts, and fibrosis by HE.Results:
This study showed a higher inflammatory reaction in TEG than CG inflammatory cell-count (CG 1.07±0.9; TEG 8.61±0.36, p<0.001); fibroblast count (CG 2.92±0.17; TEG 19.14±0.62, p<0.001), and fibrosis quantification (CG 2.0; TEG 3.75, p<0.001). The analysis of the HA volume in nine weeks in the dorsal region did not show a difference between groups (p=0.39).Conclusions:
This study suggested that the HA injection in the TEG caused an increase in inflammatory cell count, fibroblast, and fibrosis quantification when compared to the CG. There was no difference in the duration of effect of HA between the groups.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tobacco
/
Inhalation Exposure
/
Viscosupplements
/
Fibroblasts
/
Hyaluronic Acid
/
Inflammation
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2019
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
USP/BR
/
Universidade de São Paulo/BR
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