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Effect of low power laser in biomodulation of cultured osteoblastic cells of Wistar rats
Morsoleto, Maria Jose Misael da Silva; Sella, Valeria; Machado, Paula; Bomfim, Fernando do; Fernandes, Maria Helena; Morgado, Fernando; Lopes Filho, Gaspar de Jesus; Plapler, Helio.
  • Morsoleto, Maria Jose Misael da Silva; Universidade Federal de São Paulo. Postgraduate Program in Interdisciplinary Surgical Sciences. BR
  • Sella, Valeria; UNIFESP. Postgraduate Program in Interdisciplinary Surgical Science. Sao Paulo. BR
  • Machado, Paula; UNIFESP. Postgraduate Program in Interdisciplinary Surgical Sciences. Sao Paulo. BR
  • Bomfim, Fernando do; UNIFESP. Postgraduate Program in Interdisciplinary Surgical Sciences. Sao Paulo. BR
  • Fernandes, Maria Helena; Universidade do Porto. Dental Medicine Faculty. Department of Pharmacology and Cellular Compatibility. PT
  • Morgado, Fernando; Universidade de Aveiro. Department of Biology. PT
  • Lopes Filho, Gaspar de Jesus; UNIFESP. Medical School. Department of Surgery. Sao Paulo. BR
  • Plapler, Helio; UNIFESP. Medical School. Department of Surgery. Sao Paulo. BR
Acta cir. bras ; 34(2): e201900210, 2019. tab, graf
Article in English | LILACS | ID: biblio-989058
ABSTRACT
Abstract

Purpose:

To analyze aspects of the biomodulating effect of light in biological tissues, bone cells from surgical explants of the femur of rats were irradiated with low intensity laser.

Methods:

Bone cells were cultured and irradiated with LASER light (GaAlAs). Growth, cell viability, mineralized matrix formation, total protein dosage, immunostimulatory properties, cytochemical analysis, gene expression of bone proteins were examined using live cell imaging and cell counting by colorimetric assay. The gene expression of alkaline phosphatase (ALP), type 1 collagen, osteocalcin and osteopontin through the real-time polymerase chain reaction.

Results:

At 8 days, the viability of the irradiated culture was 82.3% and 72.4% in non-irradiated cells. At 18 days, the cellular viability (with laser) was 77.42% and 47.62% without laser. At 8 days, the total protein concentration was 21.622 mg / mol in the irradiated group and 16, 604 mg / mol in the non-irradiated group and at 18 days the concentration was 37.25 mg / mol in the irradiated group and 24, 95 mg / mol in the non-irradiated group.

Conclusion:

The laser interfered in the histochemical reaction, cell viability, matrix mineralization, and maintained the cellular expression of proteins
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Osteoblasts / Cell Differentiation / Cell Survival / Low-Level Light Therapy Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: Brazil / Portugal Institution/Affiliation country: UNIFESP/BR / Universidade Federal de São Paulo/BR / Universidade de Aveiro/PT / Universidade do Porto/PT

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Full text: Available Index: LILACS (Americas) Main subject: Osteoblasts / Cell Differentiation / Cell Survival / Low-Level Light Therapy Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: Brazil / Portugal Institution/Affiliation country: UNIFESP/BR / Universidade Federal de São Paulo/BR / Universidade de Aveiro/PT / Universidade do Porto/PT